Composition and diversity of endophytic bacterial communities in the tubers of Pinellia ternata from different regions and their effects on succinate biosynthesis based on high-throughput sequencing.

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-06 DOI:10.1007/s13205-024-04108-1
Peng Zhang, Wei Ding, Heng Zheng
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引用次数: 0

Abstract

In this study, high-throughput sequencing (HTS) technology was used to investigate the composition and diversity of endophytic bacteria and their effects on succinic acid biosynthesis in P. ternata tubers from three different geographical locations (MS, SL, and ZT). A total of 1777 amplicon sequence variants (ASVs) were annotated, and the diversity and composition of endophytic bacteria in P. ternata tubers were significantly different among different regions. The ZT samples presented the highest α diversity, and the Shannon diversity, richness, and Pielou evenness index were all ZT > MS > SL. Co-occurrence network analysis revealed that endophytic bacterial groups such as Stenotrophomonas, Pseudomonas, Mycobacterium, and Chryseomicrobium were key groups in the endophytic bacterial interaction network, indicating that they play a role in maintaining community stability. In addition, some endophytic bacteria were associated with the biosynthesis of succinic acid, a key bioactive compound in P. ternata. The succinate content was positively correlated with the genera Brevundimonas, Ensifer, Nocardioides, and Paenibacillus, while it was negatively correlated with the genera Lentimicrobium, Anaerovorax, and Pajaroellobacter. These findings highlight the key role of endophytic bacteria in regulating the efficacy of P. ternata. These findings provide key information for further elucidating the mechanism by which endophytic bacteria affect the synthesis of bioactive compounds.

基于高通量测序的不同地区半夏块茎内生细菌群落的组成和多样性及其对琥珀酸生物合成的影响。
本研究采用高通量测序(HTS)技术研究了三个不同地理位置(MS、SL和ZT)的P. ternata块茎中内生细菌的组成和多样性及其对琥珀酸生物合成的影响。共注释了 1777 个扩增子序列变异(ASVs),不同地区 P. ternata 块茎中内生细菌的多样性和组成存在显著差异。ZT 样品的 α 多样性最高,香农多样性、丰富度和 Pielou 均匀度指数均为 ZT > MS > SL。共生网络分析显示,内生细菌群(如 Stenotrophomonas、Pseudomonas、Mycobacterium 和 Chryseomicrobium)是内生细菌相互作用网络中的关键群,表明它们在维持群落稳定性方面发挥作用。此外,一些内生细菌与琥珀酸的生物合成有关,琥珀酸是 P. ternata 的一种关键生物活性化合物。琥珀酸含量与 Brevundimonas 属、Ensifer 属、Nocardioides 属和 Paenibacillus 属呈正相关,而与 Lentimicrobium 属、Anaerovorax 属和 Pajaroellobacter 属呈负相关。这些发现凸显了内生细菌在调节 P. ternata 的功效方面所起的关键作用。这些发现为进一步阐明内生细菌影响生物活性化合物合成的机制提供了关键信息。
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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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