Sublethal effects of lambda-cyhalothrin on the biological characteristics, detoxification enzymes, and genes of the papaya mealybug, Paracoccus marginatus
Jian-Yu Li, Yan-Ting Chen, Yan Chen, Jian-Wei Zhao, Jian-Wei Fu, Meng-Zhu Shi
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引用次数: 0
Abstract
The papaya mealybug, Paracoccus marginatus, is an invasive pest that causes significant damage to various tropical fruits and ornamentals worldwide. Lambda-cyhalothrin, an effective insecticide in integrated pest management (IPM) strategies, can control P. marginatus. This study evaluated the sublethal effect of lambda-cyhalothrin on the life table parameters and the detoxification enzymes activities of P. marginatus. Furthermore, transcriptome sequencing analysis revealed the potential mechanisms of the detoxification-related genes associated with enzymes activities. The study reported that lambda-cyhalothrin showed high activity against P. marginatus, with LC30 values of 8.98 mg/L. Two-sex life table results showed that the development duration of the second instar nymphs, preadults, and adult female longevity was significantly prolonged, and the fecundity was stimulated in the F1-CY of P. marginatus when the parental generation was exposed to LC30 lambda-cyhalothrin. Moreover, lambda-cyhalothrin treatment significantly increased the activities of Cytochrome P450 monooxygenases (P450) and glutathione-S-transferase (GST) enzymes. Additionally, significant expression levels of P450, GST, carboxylesterase, and esterase genes were observed in P. marginatus subjected to the sublethal lambda-cyhalothrin exposure. Our findings demonstrate that sublethal concentrations of lambda-cyhalothrin influenced the development and fecundity of P. marginatus, and changes in enzymes activities and differential expression of detoxifying genes might be involved in the response and detoxification metabolism of lambda-cyhalothrin in P. marginatus. These results could contribute to a comprehensive risk assessment and provide guidance for the scientific use of lambda-cyhalothrin in IPM strategies.
期刊介绍:
Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues.
Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates.
Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management.
Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.