Xyloglucan endotransglucosylase-hydrolase 1 is a negative regulator of drought tolerance in barley via modulating lignin biosynthesis and stomatal closure

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-10-05 DOI:10.1016/j.plaphy.2024.109171

Man-Man Fu , Fangbin Cao , Cheng-Wei Qiu , Chen Liu , Tao Tong , Xue Feng , Shengguan Cai , Zhong-Hua Chen , Feibo Wu

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引用次数: 0

Abstract

The projected increase in drought severity and duration worldwide poses a significant threat to crop growth and sustainable food production. Xyloglucan endotransglucosylase/hydrolases (XTHs) family is essential in cell wall modification through the construction and restructuring of xyloglucan cross-links, but their role in drought tolerance and stomatal regulation is still illusive. We cloned and functionally characterized HvXTH1 using genetic, physiological, biochemical, transcriptomic and metabolomic approaches in barley. Evolutionary bioinformatics showed that orthologues of XTH1 was originated from Streptophyte algae (e.g. some species in the Zygnematales) the closest clade to land plants based on OneKP database. HvXTH1 is highly expressed in leaves and HvXTH1 is localized to the plasma membrane. Under drought conditions, silencing HvXTH1 in drought-tolerant Tibetan wild barley XZ5 induced a significant reduction in water loss rate and increase in biomass, however overexpressing HvXTH1 exhibited drought sensitivity with significantly less drought-responsive stomata, lower lignin content and a thicker cell wall. Transcriptome profile of the wild type Golden Promise and HvXTH1-OX demonstrated that drought-induced differentially expressed genes in leaves are related to cell wall biosynthesis, abscisic acid and stomatal signaling, and stress response. Furthermore, overexpressing HvXTH1 suppressed both genes and metabolites in the phenylpropanoid pathway for lignin biosynthesis, leading to drought sensitivity of HvXTH1-OX. We provide new insight by deciphering the function of a novel protein HvXTH1 for drought tolerance in cell wall modification, stomatal regulation, and phenylpropanoid pathway for lignin biosynthesis in barley. The function of HvXTH1 in drought response will be beneficial to develop crop varieties adapted to drought.

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木聚糖内转糖基酶-水解酶1是大麦耐旱性的负调控因子，它通过调节木质素的生物合成和气孔关闭来提高大麦的耐旱性。

全球干旱的严重程度和持续时间预计会增加，这对作物生长和可持续粮食生产构成了重大威胁。木聚糖内转糖基酶/水解酶（XTHs）家族通过构建和重组木聚糖交联在细胞壁修饰中起着至关重要的作用，但它们在耐旱性和气孔调控中的作用仍不明确。我们利用遗传、生理、生化、转录组学和代谢组学方法克隆了大麦中的 HvXTH1，并对其进行了功能表征。生物信息学进化研究表明，根据 OneKP 数据库，XTH1 的直向同源物起源于链格藻（如 Zygnematales 中的一些物种），而链格藻是与陆地植物最接近的支系。HvXTH1 在叶片中高表达，并且定位于质膜。在干旱条件下，对耐旱的西藏野生青稞XZ5沉默HvXTH1可显著降低失水率并增加生物量，而过表达HvXTH1则表现出对干旱的敏感性，对干旱反应的气孔显著减少，木质素含量降低，细胞壁变厚。野生型 Golden Promise 和 HvXTH1-OX 的转录组图谱表明，干旱诱导的叶片差异表达基因与细胞壁生物合成、脱落酸和气孔信号转导以及胁迫响应有关。此外，过表达 HvXTH1 会抑制木质素生物合成的苯丙酮途径中的基因和代谢产物，从而导致 HvXTH1-OX 对干旱的敏感性。我们通过破译新型蛋白质 HvXTH1 在大麦细胞壁修饰、气孔调控和木质素生物合成的苯丙酮途径中的抗旱功能，提供了新的见解。HvXTH1在干旱响应中的功能将有利于培育适应干旱的作物品种。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.