Transcription factor NtMYB59 targets NtMYB12 to negatively regulate the biosynthesis of polyphenols in Nicotiana tabacum

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-10-05 DOI:10.1016/j.plaphy.2024.109181

Chi Zhang , Yongbin Liu , Yali Liu , Hongguang Li , Yudong Chen , Bingyu Li , Shun He , Qiansi Chen , Jun Yang , Qian Gao , Zhong Wang

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引用次数: 0

Abstract

MYB12 is a key regulator that has been shown to promote the accumulation of various phenylpropanoid compounds in plants. However, the regulation of MYB12 gene is largely unknown. In this study, we found that overexpression of the NtMYB59 gene significantly inhibited the accumulation of chlorogenic acid (CGA), flavonols, and anthocyanins in tobacco, while knock-down and knock-out of NtMYB59 significantly increased the contents of these polyphenol compounds. Transcriptome analysis between WT and NtMYB59-OE plants revealed several differentially expressed genes (DEGs) encoding crucial enzymes in the phenylpropanoid pathway and the transcription factor NtMYB12. ChIP-seq assay further indicated that NtMYB12 might be a direct target of NtMYB59. Subsequent yeast one-hybrid, electrophoretic mobility shift assay, and Dual-Luciferase assays confirmed that NtMYB59 directly binds to the promoter of NtMYB12 to inhibit its expression. Moreover, loss-function of NtMYB59 significantly promoted the accumulation of flavonols and anthocyanins in ntmyb59, but their contents in ntmyb59/ntmyb12 double mutants were significantly lower than that of WT and ntmyb59 plants, indicating that the regulation of NtMYB59 on flavonoids biosynthesis depends on the activity of NtMYB12. Our study revealed that NtMYB59 regulates the expression of NtMYB12, and provided new potential strategies for modulating phenylpropanoids biosynthesis in tobacco.

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转录因子 NtMYB59 以 NtMYB12 为靶标，负向调节烟草中多酚的生物合成。

MYB12 是一个关键的调控因子，已被证明能促进植物中各种苯丙类化合物的积累。然而，MYB12 基因的调控机制尚不清楚。本研究发现，过表达 NtMYB59 基因会显著抑制烟草中绿原酸（CGA）、黄酮醇和花青素的积累，而敲除和剔除 NtMYB59 基因会显著增加这些多酚化合物的含量。对 WT 和 NtMYB59-OE 植物的转录组分析发现了几个差异表达基因（DEGs），这些基因编码苯丙醇途径中的关键酶和转录因子 NtMYB12。ChIP-seq 分析进一步表明，NtMYB12 可能是 NtMYB59 的直接靶标。随后的酵母单杂交、电泳迁移实验和双荧光素酶实验证实，NtMYB59 直接与 NtMYB12 的启动子结合，抑制其表达。此外，NtMYB59功能缺失可显著促进ntmyb59中黄酮醇和花青素的积累，但在ntmyb59/ntmyb12双突变体中，黄酮醇和花青素的含量显著低于WT和ntmyb59植株，表明NtMYB59对黄酮类化合物生物合成的调控依赖于NtMYB12的活性。我们的研究揭示了NtMYB59调控NtMYB12的表达，为调控烟草中苯丙酮类化合物的生物合成提供了新的潜在策略。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.