Prevalence and characteristics of 11 potentially diarrhoeagenic microbes in asymptomatic individuals in Norway, 2015–2020

IF 2.2 4区 医学 Q4 IMMUNOLOGY
Apmis Pub Date : 2024-10-06 DOI:10.1111/apm.13478
Einar Tollaksen Weme, Lin Thorstensen Brandal, Pål Arne Jenum, Astrid Louise Wester, Fredrik Müller
{"title":"Prevalence and characteristics of 11 potentially diarrhoeagenic microbes in asymptomatic individuals in Norway, 2015–2020","authors":"Einar Tollaksen Weme,&nbsp;Lin Thorstensen Brandal,&nbsp;Pål Arne Jenum,&nbsp;Astrid Louise Wester,&nbsp;Fredrik Müller","doi":"10.1111/apm.13478","DOIUrl":null,"url":null,"abstract":"<p>We aimed to estimate the prevalence of potentially diarrhoeagenic microbes (PDMs) in faecal samples from asymptomatic individuals in a high-income country, identify risk factors for carriage and to identify microbial factors that differ between PDMs in asymptomatic versus symptomatic individuals. Samples from 1000 asymptomatic participants were collected, together with a questionnaire, between 2015 and 2020 and examined by PCR for 11 PDMs. Isolates were characterised and potential risk factors were registered. Atypical enteropathogenic <i>Escherichia coli</i> (aEPEC), <i>Yersinia enterocolitica</i>, Shiga toxin-producing <i>E. coli</i> (STEC), enterotoxigenic <i>E. coli</i> (ETEC) and <i>Campylobacter</i> spp. were found in 163 (16%), 20 (2.0%), 17 (1.7%), 12 (1.2%) and 11 (1.1%) asymptomatic individuals, respectively. Other PDMs were rare. Only low virulent STEC, with <i>stx1c</i>, <i>stx2b</i> or <i>stx2f</i>, was detected. Travels outside Europe was a significant risk factor for detecting <i>Campylobacter</i> spp. (odds ratio (OR) 6.99; 95% CI 1.12–43.6) and ETEC (OR 11.4; 95% CI 1.26–102). Individuals ≥65 years of age had lower odds of carrying STEC (OR 0.11; 95% CI 0.02–0.57) or EPEC (OR 0.09; 95% CI 0.05–0.16) than individuals ≤5 years of age. The common finding of PDMs in asymptomatic individuals could have implications for the interpretation of positive findings in clinical samples and infection control measures.</p>","PeriodicalId":8167,"journal":{"name":"Apmis","volume":"132 11","pages":"797-806"},"PeriodicalIF":2.2000,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apm.13478","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Apmis","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/apm.13478","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

We aimed to estimate the prevalence of potentially diarrhoeagenic microbes (PDMs) in faecal samples from asymptomatic individuals in a high-income country, identify risk factors for carriage and to identify microbial factors that differ between PDMs in asymptomatic versus symptomatic individuals. Samples from 1000 asymptomatic participants were collected, together with a questionnaire, between 2015 and 2020 and examined by PCR for 11 PDMs. Isolates were characterised and potential risk factors were registered. Atypical enteropathogenic Escherichia coli (aEPEC), Yersinia enterocolitica, Shiga toxin-producing E. coli (STEC), enterotoxigenic E. coli (ETEC) and Campylobacter spp. were found in 163 (16%), 20 (2.0%), 17 (1.7%), 12 (1.2%) and 11 (1.1%) asymptomatic individuals, respectively. Other PDMs were rare. Only low virulent STEC, with stx1c, stx2b or stx2f, was detected. Travels outside Europe was a significant risk factor for detecting Campylobacter spp. (odds ratio (OR) 6.99; 95% CI 1.12–43.6) and ETEC (OR 11.4; 95% CI 1.26–102). Individuals ≥65 years of age had lower odds of carrying STEC (OR 0.11; 95% CI 0.02–0.57) or EPEC (OR 0.09; 95% CI 0.05–0.16) than individuals ≤5 years of age. The common finding of PDMs in asymptomatic individuals could have implications for the interpretation of positive findings in clinical samples and infection control measures.

2015-2020 年挪威无症状人群中 11 种潜在致腹泻微生物的流行率和特征。
我们的目的是估算高收入国家无症状人群粪便样本中潜在致泻微生物(PDMs)的流行率,确定携带的风险因素,并找出无症状人群与有症状人群中 PDMs 不同的微生物因素。研究人员在 2015 年至 2020 年期间收集了 1000 名无症状参与者的样本和调查问卷,并通过 PCR 检测了 11 种 PDM。对分离菌进行了定性,并登记了潜在的风险因素。在163名(16%)、20名(2.0%)、17名(1.7%)、12名(1.2%)和11名(1.1%)无症状者中分别发现了非典型肠致病性大肠杆菌(aEPEC)、小肠结肠耶尔森菌、产志贺毒素大肠杆菌(STEC)、肠毒性大肠杆菌(ETEC)和弯曲杆菌属。其他病原微生物很少见。只检出了stx1c、stx2b或stx2f型低毒性STEC。欧洲以外的旅行是检出弯曲杆菌属(几率比(OR)6.99;95% CI 1.12-43.6)和 ETEC(OR 11.4;95% CI 1.26-102)的重要风险因素。年龄≥65 岁者携带 STEC(OR 0.11;95% CI 0.02-0.57)或 EPEC(OR 0.09;95% CI 0.05-0.16)的几率低于年龄≤5 岁者。在无症状者中发现 PDMs 的情况很常见,这可能会对临床样本中阳性结果的解释和感染控制措施产生影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Apmis
Apmis 医学-病理学
CiteScore
5.20
自引率
0.00%
发文量
91
审稿时长
2 months
期刊介绍: APMIS, formerly Acta Pathologica, Microbiologica et Immunologica Scandinavica, has been published since 1924 by the Scandinavian Societies for Medical Microbiology and Pathology as a non-profit-making scientific journal.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信