Diagnostic value of IFN-gamma in tuberculous pleural effusion

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine Pub Date : 2024-10-05 DOI:10.1016/j.cyto.2024.156773

Hongchun Huang , Yonghuai Li , Xiaohui Cao , Minghui Yang , Jilu Shen

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引用次数: 0

Abstract

Background

Simple, rapid, and accurate diagnosis of tuberculous pleural effusion (TPE) remains challenging. This study aimed to determine the accuracy of IFN-γ in diagnosing TPE.

Methods

We quantified the expression of interferon-gamma (IFN-γ) in blood (B), adenosine deaminase (ADA), and IFN-γ in pleural effusions (PE) from 25 TPE patients and 31 non-TPE patients using a combination of immunological assays and flow cytometric analysis. The diagnostic performance of these three biomarkers was evaluated using receiver operating characteristic (ROC) curves.

Results

We found that IFN-γ levels in blood and pleural fluid were higher in the TPE group than in the non-TPE group. The mean concentration of IFN-γ in pleural fluid of the TPE group was 3140.90 (1817.94, 6611.05) pg/mL, while that of the non-TPE group was 4.91 (0.69, 8.6) pg/mL), and the difference was statistically significant (z = 6.39, P < 0.001). The mean blood IFN-γ was 40.19 (16.45, 59.08) pg/mL in the TPE group and 2.76 (1.96, 6.02) pg/mL in the non-TPE group, which was statistically different (z = 5.12, P < 0.001). The area under the ROC curve (AUC) for pleural fluid IFN-γ, blood IFN-γ, and ADA were 0.999 (95 % CI: 0.994–1.00), 0.901 (95 % CI: 0.798–1.00) and 0.996 (95 % CI: 0.987–1.00), respectively.

Conclusion

This study confirms that IFN-γ has high diagnostic validity in patients with TPE and can potentially be an excellent biomarker.

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IFN-gamma 在结核性胸腔积液中的诊断价值。

背景：简单、快速、准确地诊断结核性胸腔积液（TPE）仍具有挑战性。本研究旨在确定 IFN-γ 诊断 TPE 的准确性：我们采用免疫学测定和流式细胞分析相结合的方法，对 25 名 TPE 患者和 31 名非 TPE 患者的血液（B）、腺苷脱氨酶（ADA）和胸腔积液（PE）中γ干扰素（IFN-γ）的表达进行了量化。我们使用接收器操作特征曲线（ROC）评估了这三种生物标记物的诊断性能：结果：我们发现，TPE 组患者血液和胸腔积液中的 IFN-γ 水平高于非 TPE 组。TPE 组胸腔积液中 IFN-γ 的平均浓度为 3140.90 (1817.94, 6611.05) pg/mL，而非 TPE 组为 4.91 (0.69, 8.6) pg/mL，差异有统计学意义（z = 6.39，P 结论：本研究证实，TPE 组胸腔积液中的 IFN-γ 浓度高于非 TPE 组：本研究证实，IFN-γ 在 TPE 患者中具有很高的诊断有效性，有可能成为一种很好的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytokine 医学-免疫学

CiteScore

7.60

自引率

2.60%

发文量

262

审稿时长

48 days

期刊介绍： The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.