Associations of age and sex with characteristics of extracellular vesicles and protein-enriched fractions of blood plasma.

IF 8 1区医学 Q1 CELL BIOLOGY

Aging Cell Pub Date : 2024-10-07 DOI:10.1111/acel.14356

Yiyao Huang, Junjie Feng, Jiannan Xu, Liang Dong, Wanting Su, Bo Li, Kenneth W Witwer, Lei Zheng

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引用次数: 0

Abstract

Extracellular vesicles (EVs) are nanosized particles that are released by various cell types and play vital roles in intercellular communication. They carry biological molecules reflecting the physiological and pathological states of their source cells and tissues, showing potential as biomarkers. However, the impact of demographic factors like age and sex on the properties of blood plasma EVs remains underexplored. This study aims to fill this gap by evaluating how these factors influence the particle count and proteomic profiles of plasma EV preparations and corresponding protein fractions. Plasma samples from 120 healthy volunteers were collected and pooled into six groups: young males (age: 27.6 ± 4.0), young females (27.4 ± 3.8), middle-aged males (48.8 ± 3.8), middle-aged females (48.9 ± 3.9), old males (69.3 ± 3.9), and old females (69.4 ± 4.3). EV- and protein-enriched fractions were separated by size-exclusion chromatography (SEC). Fractions were characterized for particle number concentration and protein composition to identify characteristics affected by age and biological sex. Plasma EVs and corresponding protein fractions exhibited distinct characteristics, with differential enrichment of markers related to EVs and other blood components, including lipoproteins. Proteomic profiles of both EVs and protein fractions displayed sex- and age-dependent differences. Differentially abundant proteins displayed functions previously identified in the context of aging and sex differences, highlighting their utility as biomarkers. Age and sex significantly affect the characteristics of plasma EVs and proteins, potentially influencing their efficacy and interpretation as biomarkers in clinical applications. This study lays the groundwork for detailed mechanistic research to understand how EVs mediate age- and sex-related effects in health.

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年龄和性别与血浆中细胞外囊泡和富含蛋白质组分特征的关系

细胞外囊泡（EVs）是由各种类型细胞释放的纳米级颗粒，在细胞间通信中发挥着重要作用。它们携带的生物分子反映了其来源细胞和组织的生理和病理状态，具有作为生物标记物的潜力。然而，年龄和性别等人口统计学因素对血浆EVs特性的影响仍未得到充分探索。本研究旨在通过评估这些因素如何影响血浆EV制备物和相应蛋白质组分的粒子数和蛋白质组学特征来填补这一空白。研究人员收集了 120 名健康志愿者的血浆样本，并将其分成六组：年轻男性（年龄：27.6 ± 4.0）、年轻女性（27.4 ± 3.8）、中年男性（48.8 ± 3.8）、中年女性（48.9 ± 3.9）、老年男性（69.3 ± 3.9）和老年女性（69.4 ± 4.3）。用大小排阻色谱法（SEC）分离了富含EV和蛋白质的馏分。对馏分的颗粒数浓度和蛋白质组成进行表征，以确定受年龄和生物性别影响的特征。血浆EV和相应的蛋白质馏分表现出不同的特征，与EV和其他血液成分（包括脂蛋白）相关的标记物富集程度不同。EVs和蛋白质组分的蛋白质组图谱显示出与性别和年龄有关的差异。富集程度不同的蛋白质显示了以前在衰老和性别差异中发现的功能，突出了它们作为生物标记物的效用。年龄和性别会明显影响血浆 EVs 和蛋白质的特征，从而可能影响它们作为生物标记物在临床应用中的功效和解释。这项研究为详细的机理研究奠定了基础，以了解 EV 如何介导与年龄和性别相关的健康影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aging Cell Biochemistry, Genetics and Molecular Biology-Cell Biology

自引率

2.60%

发文量

212

期刊介绍： Aging Cell is an Open Access journal that focuses on the core aspects of the biology of aging, encompassing the entire spectrum of geroscience. The journal's content is dedicated to publishing research that uncovers the mechanisms behind the aging process and explores the connections between aging and various age-related diseases. This journal aims to provide a comprehensive understanding of the biological underpinnings of aging and its implications for human health. The journal is widely recognized and its content is abstracted and indexed by numerous databases and services, which facilitates its accessibility and impact in the scientific community. These include: Academic Search (EBSCO Publishing) Academic Search Alumni Edition (EBSCO Publishing) Academic Search Premier (EBSCO Publishing) Biological Science Database (ProQuest) CAS: Chemical Abstracts Service (ACS) Embase (Elsevier) InfoTrac (GALE Cengage) Ingenta Select ISI Alerting Services Journal Citation Reports/Science Edition (Clarivate Analytics) MEDLINE/PubMed (NLM) Natural Science Collection (ProQuest) PubMed Dietary Supplement Subset (NLM) Science Citation Index Expanded (Clarivate Analytics) SciTech Premium Collection (ProQuest) Web of Science (Clarivate Analytics) Being indexed in these databases ensures that the research published in Aging Cell is discoverable by researchers, clinicians, and other professionals interested in the field of aging and its associated health issues. This broad coverage helps to disseminate the journal's findings and contributes to the advancement of knowledge in geroscience.