Integration of Fe-MOF-laccase-magnetic biochar: From Rational Designing of a biocatalyst to aflatoxin B1 decontamination of peanut oil

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere Pub Date : 2024-11-01 DOI:10.1016/j.chemosphere.2024.143424

Usman Rasheed , Qurat Ul Ain , Bin Liu

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Abstract

Enzymatic degradation of aflatoxins in food commodities has gained significant attention. However, enzyme denaturation in organic media discourages their direct use in oils to remove aflatoxins. For that, enzymes are immobilized or encapsulated for improved stability and reusability under unfavorable conditions. We sandwiched the laccase between a carrier and an outer protective layer. We used spent-mushroom-substrate (SMS) derived porous magnetic biochar as the laccase carrier and coated it with an iron MOF to create a biocomposite, Fe-BTC@Lac@FB. The immobilized laccase demonstrated enhanced chemical, thermal, and storage stability and proficient reusability. Fe-BTC@Lac@FB exhibited 11 times enhanced aflatoxin B₁ (AFB₁) degradation compared to free laccase (FL). In addition, thermally inactivated Fe-BTC@Lac@FB could adsorb 11.2 mg/g of AFB₁ from peanut oil. Multi-aflatoxin removal also proved promising, while Fe-BTC@Lac@FB could retain >85 % of AFB₁ removal efficacy after five reusability cycles. Fe-BTC@Lac@FB treatment did not affect peanut oil quality as indicated by different oil quality parameters and proved essentially non-cytotoxic. All these aspects helped recognize Fe-BTC@Lac@FB as an excellent laccase-carrying material with exceptionally higher stability, activity, and reusability.

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Fe-MOF-漆酶-磁性生物炭的整合：从合理设计生物催化剂到花生油中黄曲霉毒素 B1 的净化。

酶法降解食品中的黄曲霉毒素已受到广泛关注。然而，酶在有机介质中变性，不利于直接用于油类中去除黄曲霉毒素。为此，需要对酶进行固定或封装，以提高其稳定性和在不利条件下的重复使用性。我们将漆酶夹在载体和外保护层之间。我们使用废蘑菇基质（SMS）衍生的多孔磁性生物炭作为漆酶载体，并在其上涂覆铁MOF，从而创建了一种生物复合材料--Fe-BTC@Lac@FB。固定化的漆酶具有更高的化学、热和贮存稳定性以及良好的重复使用性。与游离漆酶（FL）相比，Fe-BTC@Lac@FB 对黄曲霉毒素 B1（AFB1）的降解能力提高了 11 倍。此外，热失活的 Fe-BTC@Lac@FB 能从花生油中吸附 11.2 mg/g 的 AFB1。事实证明，Fe-BTC@Lac@FB 对多种黄曲霉毒素也有很好的去除效果，经过五个重复使用周期后，Fe-BTC@Lac@FB 对 AFB1 的去除率仍大于 85%。从不同的油质参数来看，Fe-BTC@Lac@FB 处理不会影响花生油的质量，而且基本上没有细胞毒性。所有这些方面都表明，Fe-BTC@Lac@FB 是一种具有极高稳定性、活性和可重复使用性的优秀漆包线杆菌载体材料。

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来源期刊

Chemosphere 环境科学-环境科学

CiteScore

15.80

自引率

8.00%

发文量

4975

审稿时长

3.4 months

期刊介绍： Chemosphere, being an international multidisciplinary journal, is dedicated to publishing original communications and review articles on chemicals in the environment. The scope covers a wide range of topics, including the identification, quantification, behavior, fate, toxicology, treatment, and remediation of chemicals in the bio-, hydro-, litho-, and atmosphere, ensuring the broad dissemination of research in this field.