Unveiling placental development in circadian rhythm-disrupted mice: A photo-acoustic imaging study on unstained tissue

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta Pub Date : 2024-10-02 DOI:10.1016/j.placenta.2024.10.001

{"title":"Unveiling placental development in circadian rhythm-disrupted mice: A photo-acoustic imaging study on unstained tissue","authors":"","doi":"10.1016/j.placenta.2024.10.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>Circadian rhythm disruption has garnered significant attention for its adverse effects on human health, particularly in reproductive medicine and fetal well-being. Assessing pregnancy health often relies on diagnostic markers such as the labyrinth zone (LZ) proportion within the placenta. This study aimed to investigate the impact of disrupted circadian rhythms on placental health and fetal development using animal models.</div></div><div><h3>Methods and results</h3><div>Employing unstained photo-acoustic microscopy (PAM) and hematoxylin and eosin (HE)-stained images, we found them mutually reinforcing. Our images revealed the role of maternal circadian rhythm disrupted group (MCRD) on the LZ and fetus weight: a decrease in LZ area from 5.01 (4.25) mm<sup>2</sup> HE (PAM) to 3.58 (2.62) mm<sup>2</sup> HE (PAM) on day 16 and 6.48 (5.16) mm<sup>2</sup> HE (PAM) to 4.61 (3.03) mm<sup>2</sup> HE (PAM) on day 18, resulting in 0.71 times lower fetus weights. We have discriminated a decrease in the mean LZ to placenta area ratio from 64 % to 47 % on day 18 in mice with disrupted circadian rhythms with PAM.</div></div><div><h3>Discussion</h3><div>The study highlights the negative influence of circadian rhythm disruption on placental development and fetal well-being. Reduced LZ area and fetal weights in the MCRD group suggest compromised placental function under disrupted circadian rhythms. PAM imaging proved to be an efficient technique for assessing placental development, offering advantages over traditional staining methods. These findings contribute to understanding the underlying mechanisms of circadian disruption on reproductive health and fetal development. Further research is needed to explore interventions to mitigate these effects and improve pregnancy outcomes.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Placenta","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0143400424006647","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction

Circadian rhythm disruption has garnered significant attention for its adverse effects on human health, particularly in reproductive medicine and fetal well-being. Assessing pregnancy health often relies on diagnostic markers such as the labyrinth zone (LZ) proportion within the placenta. This study aimed to investigate the impact of disrupted circadian rhythms on placental health and fetal development using animal models.

Methods and results

Employing unstained photo-acoustic microscopy (PAM) and hematoxylin and eosin (HE)-stained images, we found them mutually reinforcing. Our images revealed the role of maternal circadian rhythm disrupted group (MCRD) on the LZ and fetus weight: a decrease in LZ area from 5.01 (4.25) mm² HE (PAM) to 3.58 (2.62) mm² HE (PAM) on day 16 and 6.48 (5.16) mm² HE (PAM) to 4.61 (3.03) mm² HE (PAM) on day 18, resulting in 0.71 times lower fetus weights. We have discriminated a decrease in the mean LZ to placenta area ratio from 64 % to 47 % on day 18 in mice with disrupted circadian rhythms with PAM.

Discussion

The study highlights the negative influence of circadian rhythm disruption on placental development and fetal well-being. Reduced LZ area and fetal weights in the MCRD group suggest compromised placental function under disrupted circadian rhythms. PAM imaging proved to be an efficient technique for assessing placental development, offering advantages over traditional staining methods. These findings contribute to understanding the underlying mechanisms of circadian disruption on reproductive health and fetal development. Further research is needed to explore interventions to mitigate these effects and improve pregnancy outcomes.

查看原文本刊更多论文

揭示昼夜节律紊乱小鼠的胎盘发育过程：对未染色组织的光声成像研究

导言：昼夜节律紊乱对人类健康，尤其是生殖医学和胎儿健康的不利影响已引起人们的极大关注。评估妊娠健康通常依赖于胎盘内迷宫区（LZ）比例等诊断指标。本研究旨在利用动物模型研究昼夜节律紊乱对胎盘健康和胎儿发育的影响：我们采用未染色的光声显微镜（PAM）和苏木精及伊红（HE）染色的图像，发现它们相互促进。我们的图像显示了母体昼夜节律紊乱组（MCRD）对LZ和胎儿体重的影响：第16天，LZ面积从5.01（4.25）mm2 HE（PAM）降至3.58（2.62）mm2 HE（PAM）；第18天，LZ面积从6.48（5.16）mm2 HE（PAM）降至4.61（3.03）mm2 HE（PAM），导致胎儿体重降低0.71倍。我们发现，在昼夜节律紊乱的小鼠中，第18天LZ与胎盘的平均面积比从64%降至47%：本研究强调了昼夜节律紊乱对胎盘发育和胎儿健康的负面影响。MCRD组LZ面积和胎儿体重的减少表明，在昼夜节律紊乱的情况下，胎盘功能会受到损害。事实证明，PAM成像是一种评估胎盘发育的有效技术，与传统染色法相比具有优势。这些发现有助于了解昼夜节律紊乱对生殖健康和胎儿发育的潜在影响机制。还需要进一步的研究来探索减轻这些影响和改善妊娠结局的干预措施。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Placenta 医学-发育生物学

CiteScore

6.30

自引率

10.50%

发文量

391

审稿时长

78 days

期刊介绍： Placenta publishes high-quality original articles and invited topical reviews on all aspects of human and animal placentation, and the interactions between the mother, the placenta and fetal development. Topics covered include evolution, development, genetics and epigenetics, stem cells, metabolism, transport, immunology, pathology, pharmacology, cell and molecular biology, and developmental programming. The Editors welcome studies on implantation and the endometrium, comparative placentation, the uterine and umbilical circulations, the relationship between fetal and placental development, clinical aspects of altered placental development or function, the placental membranes, the influence of paternal factors on placental development or function, and the assessment of biomarkers of placental disorders.