Cellular morphodynamics and signaling around the transcellular passage cleft during rhizobial infections of legume roots

Abstract

Legume roots allow intracellular infections of rhizobia to establish the mutualistic root nodule symbiosis. During this colonization event, specialized and membrane-defined infection threads provide the host-controlled path for the bacteria through the multilayered root tissue to reach a newly developing organ, the root nodule. On this way, bacteria have to propagate transcellularly and thus overcome cell wall barriers. This process not only requires continuous molecular surveillance of the invading microbe but also structural adaptations of the extracellular matrix components in a spatially confined manner leading to the formation of a novel compartment that we term the “transcellular passage cleft” (TPC). Here, we review the molecular mechanisms and signaling events around the TPC and propose a step-wise model for TPC formation.