Nitric oxide participates in sucrose-TOR signaling during meristem activation in Arabidopsis thaliana.

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-10-04 DOI:10.1007/s00425-024-04542-8
Ana Paula Lando, María Cecilia Terrile, María Agustina De Marco, Marianela Rodriguez, Giselle María Astrid Martínez-Noël
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Abstract

Main conclusion: This study provides evidence about the relationship between Target of Rapamycin (TOR) kinase and the signal molecule nitric oxide (NO) in plants. We showed that sucrose (SUC)-mediated TOR activation of root apical meristem (RAM) requires NO and that NO, in turn, participates in the regulation of TOR signaling. Nitric oxide (NO) constitutes a signal molecule that regulates important target proteins related to growth and development and also contributes to metabolic reprogramming that occurs under adverse conditions. Taking into account the important role of NO and its relationship with Target of Rapamycin (TOR) signaling in animals, we wondered about the putative link between both pathways in plants. With this aim, we studied a TOR-dependent process which is the reactivation of the root apical meristem (RAM) in Arabidopsis thaliana. We used pharmacological and genetic tools to evaluate the relationship between NO and TOR on the sugar induction of RAM, using SNP as NO donor, cPTIO as NO scavenger and the nitrate reductase (NR) mutant nia2. The results showed that sucrose (SUC)-mediated TOR activation of the RAM requires NO and that NO, in turn, participates in the regulation of TOR signaling. Interestingly, TOR activation induced by sugar increased the NO levels. We also observed that NO could mediate the repression of SnRK1 activity by SUC. By computational prediction we found putative S-nitrosylation sites in the TOR complex proteins and the catalytic subunit of SnRK1, SnRK1.1. The present work demonstrates for the first time a link between NO and TOR revealing the complex interplay between the two pathways in plants.

一氧化氮参与拟南芥分生组织激活过程中的蔗糖-TOR 信号转导。
主要结论本研究为雷帕霉素靶蛋白激酶(TOR)与植物体内信号分子一氧化氮(NO)之间的关系提供了证据。我们发现,蔗糖(SUC)介导的根尖分生组织(RAM)的 TOR 激活需要一氧化氮,而一氧化氮反过来又参与了 TOR 信号的调控。一氧化氮(NO)是一种信号分子,可调节与生长发育有关的重要靶蛋白,还有助于在不利条件下发生的代谢重编程。考虑到一氧化氮在动物体内的重要作用及其与雷帕霉素靶蛋白(TOR)信号转导的关系,我们想知道这两种途径在植物体内的潜在联系。为此,我们研究了拟南芥根尖分生组织(RAM)重新激活这一依赖于 TOR 的过程。我们利用药理学和遗传学工具,以 SNP 作为 NO 供体、cPTIO 作为 NO 清除剂以及硝酸还原酶(NR)突变体 nia2,评估了 NO 和 TOR 在糖诱导 RAM 过程中的关系。结果表明,蔗糖(SUC)介导的 TOR 对 RAM 的激活需要 NO,而 NO 又参与了 TOR 信号的调控。有趣的是,糖诱导的 TOR 激活增加了 NO 的水平。我们还观察到,NO 可以介导 SUC 对 SnRK1 活性的抑制。通过计算预测,我们在 TOR 复合蛋白和 SnRK1 催化亚基 SnRK1.1 中发现了推定的 S-亚硝基化位点。本研究首次证明了氮氧化物与 TOR 之间的联系,揭示了植物中这两种途径之间复杂的相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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