KAR1-dormancy release in Avena fatua caryopses includes increased AfMAN gene expression and ENDO-β-MANNANASE activity in the coleorhiza and radicle

IF 4 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology Pub Date : 2024-09-26 DOI:10.1016/j.jplph.2024.154363

Anna Orłowska, Jan Kępczyński

{"title":"KAR1-dormancy release in Avena fatua caryopses includes increased AfMAN gene expression and ENDO-β-MANNANASE activity in the coleorhiza and radicle","authors":"Anna Orłowska, Jan Kępczyński","doi":"10.1016/j.jplph.2024.154363","DOIUrl":null,"url":null,"abstract":"<div><div>KAR1, at very low concentration (3x10−9 M) released dormancy in Avena fatua caryopses, which was expressed in almost complete emergence of coleorhiza (CE) and radicle (RE) just after three days of germination. The dormancy-releasing effect of KAR1 was associated with an increased activity of ENDO-β-MANNANASE (MAN; EC 3.2.1.78) (hydrolase and transglycosylase) in coleorhiza and radicle before RE. The MAN genes, MAN1, MAN2, MAN3, MAN4, and MAN5 were for the first time identified in the genome of A. fatua. KAR1 induced expression of AfMAN1-3 and AfMAN5 in the coleorhiza and AfMAN2 and AfMAN3 in the radicle during caryopses germination. The increase in transcripts in the coleorhiza of AfMAN1,5 after 8 h and AfMAN3,5 after 12 h germination in the presence of KAR1 is probably responsible for the increase in MAN activity determined after 18 h before RE. KAR1 also increased AfMAN3 expression in radicle after 12 h which probably caused the increased MAN activity after 18 h. Therefore, release of caryopses dormancy by KAR1 involves increasing expression of MAN genes and MAN activity both in the coleorhiza and radicle, which might facilitate the passage of the radicle through the coleorhiza. The work provides the first data on the contribution of MAN, present in coleorhiza and radicle, in the dormancy release of caryopses by KAR1.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154363"},"PeriodicalIF":4.0000,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of plant physiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0176161724001949","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

KAR₁, at very low concentration (3x10⁻⁹ M) released dormancy in Avena fatua caryopses, which was expressed in almost complete emergence of coleorhiza (CE) and radicle (RE) just after three days of germination. The dormancy-releasing effect of KAR₁ was associated with an increased activity of ENDO-β-MANNANASE (MAN; EC 3.2.1.78) (hydrolase and transglycosylase) in coleorhiza and radicle before RE. The MAN genes, MAN1, MAN2, MAN3, MAN4, and MAN5 were for the first time identified in the genome of A. fatua. KAR₁ induced expression of AfMAN1-3 and AfMAN5 in the coleorhiza and AfMAN2 and AfMAN3 in the radicle during caryopses germination. The increase in transcripts in the coleorhiza of AfMAN1,5 after 8 h and AfMAN3,5 after 12 h germination in the presence of KAR₁ is probably responsible for the increase in MAN activity determined after 18 h before RE. KAR₁ also increased AfMAN3 expression in radicle after 12 h which probably caused the increased MAN activity after 18 h. Therefore, release of caryopses dormancy by KAR₁ involves increasing expression of MAN genes and MAN activity both in the coleorhiza and radicle, which might facilitate the passage of the radicle through the coleorhiza. The work provides the first data on the contribution of MAN, present in coleorhiza and radicle, in the dormancy release of caryopses by KAR₁.

查看原文本刊更多论文

Avena fatua caryopses 的 KAR1-休眠释放包括增加 AfMAN 基因的表达以及在合叶和胚根中的 ENDO-β-MANNANASE 活性。

KAR1 在极低浓度（3x10-9 M）下就能解除莜麦穗条的休眠，这表现在萌芽三天后，穗条（CE）和胚根（RE）几乎完全萌发。KAR1 的休眠释放效应与 RE 之前萌发的合叶和胚根中的ENDO-β-MANNANASE（MAN；EC 3.2.1.78）（水解酶和转糖基化酶）活性增加有关。MAN 基因 MAN1、MAN2、MAN3、MAN4 和 MAN5 是首次在 A. fatua 的基因组中发现。在核果萌发过程中，KAR1诱导AfMAN1-3和AfMAN5在合叶中的表达，AfMAN2和AfMAN3在胚根中的表达。在有 KAR1 存在的情况下，发芽 8 小时后叶鞘中 AfMAN1,5 的转录本增加，发芽 12 小时后 AfMAN3,5 的转录本增加，这可能是 RE 前 18 小时后测定的 MAN 活性增加的原因。因此，KAR1 对子叶休眠的解除涉及到增加子叶和胚根中 MAN 基因的表达和 MAN 活性，这可能会促进胚根通过子叶。该研究首次提供了KAR1释放核果休眠过程中存在于鞘叶和胚根中的MAN的贡献数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of plant physiology 生物-植物科学

CiteScore

7.20

自引率

4.70%

发文量

196

审稿时长

32 days

期刊介绍： The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.