Histone Deacetylase 3 Is Involved in Maintaining Queen Hallmarks of a Termite

IF 4.5 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Ecology Pub Date : 2024-10-04 DOI:10.1111/mec.17541

Louis Allan Okwaro, Judith Korb

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Abstract

The role of epigenetics in regulating caste polyphenism in social insects has been debated. Here, we tested the importance of histone de/acetylation processes for the maintenance of queen hallmarks like a high fecundity and a long lifespan. To this end, we performed RNA interference experiments against histone deacetylase 3 (HDAC3) in the termite Cryptotermes secundus. Fat body transcriptomes and chemical communication profiles revealed that silencing of HDAC3 leads to signals indicative of queen hallmarks. This includes fostering of queen signalling, defence against ageing and a reduction of life-shortening IIS (insulin/insulin-like growth factor signalling) and endocrine JH (juvenile hormone) signalling via Kr-h1 (Krüppel-homologue 1). These observed patterns were similar to those of a protein-enriched diet, which might imply that histone acetylation conveys nutritional effects. Strikingly, in contrast to solitary insects, reduced endocrine JH signalling had no negative effect on fecundity-related vitellogenesis in the fat bodies. This suggests an uncoupling of longevity pathways from fecundity in fat bodies, which can help explain queens' extraordinary lifespans combined with high fecundity.

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组蛋白去乙酰化酶 3 参与维持白蚁女王的特征

表观遗传学在调节社会性昆虫种姓多态性中的作用一直备受争议。在这里，我们测试了组蛋白去乙酰化过程对维持高繁殖力和长寿命等蚁后特征的重要性。为此，我们在白蚁Cryptotermes secundus中进行了针对组蛋白去乙酰化酶3（HDAC3）的RNA干扰实验。脂肪体转录组和化学通讯图谱显示，HDAC3的沉默会导致显示蚁后特征的信号。这包括促进蚁后信号传导、抵御衰老和减少缩短寿命的 IIS（胰岛素/类胰岛素生长因子信号传导）以及通过 Kr-h1（Krüppel-同源物 1）进行的内分泌 JH（幼年激素）信号传导。这些观察到的模式与富含蛋白质饮食的模式相似，这可能意味着组蛋白乙酰化传递了营养效应。令人吃惊的是，与独居昆虫相反，内分泌JH信号的减少对脂肪体中与繁殖相关的卵黄发生没有负面影响。这表明肥胖体中的长寿途径与繁殖力脱钩，这有助于解释蜂后的超长寿命与高繁殖力。

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来源期刊

Molecular Ecology 生物-进化生物学

CiteScore

8.40

自引率

10.20%

发文量

472

审稿时长

1 months

期刊介绍： Molecular Ecology publishes papers that utilize molecular genetic techniques to address consequential questions in ecology, evolution, behaviour and conservation. Studies may employ neutral markers for inference about ecological and evolutionary processes or examine ecologically important genes and their products directly. We discourage papers that are primarily descriptive and are relevant only to the taxon being studied. Papers reporting on molecular marker development, molecular diagnostics, barcoding, or DNA taxonomy, or technical methods should be re-directed to our sister journal, Molecular Ecology Resources. Likewise, papers with a strongly applied focus should be submitted to Evolutionary Applications. Research areas of interest to Molecular Ecology include: * population structure and phylogeography * reproductive strategies * relatedness and kin selection * sex allocation * population genetic theory * analytical methods development * conservation genetics * speciation genetics * microbial biodiversity * evolutionary dynamics of QTLs * ecological interactions * molecular adaptation and environmental genomics * impact of genetically modified organisms