MdNAC5: a key regulator of fructose accumulation in apple fruit

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-10-03 DOI:10.1111/nph.20158

Bo Zhang, Hui-Juan Yang, Ya-Nan Li, Zhen-Zhen Zhu, Zheng-Yang Zhao, Ya-Zhou Yang

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引用次数: 0

Abstract

The sweetness of apple fruit is a key factor in the improvement of apple varieties, with fructose being the sweetest of the soluble sugars, playing a crucial role in determining the overall sweetness of the apple. Therefore, uncovering the key genes controlling fructose accumulation and deciphering the regulatory mechanisms of fructose are vitally important for the improvement of apple varieties.
In this study, through BSA-seq and transcriptome analysis of the ‘Changfu 2’ × ‘Golden Delicious’ F1 hybrid population, MdNAC5 was identified as a key regulatory gene for fructose content.
MdNAC5 was shown to significantly influence fructose accumulation in both apples and tomatoes. Furthermore, we conducted a detailed identification of sugar transporters and metabolic enzymes in apples, discovering that MdNAC5 can enhance fructose accumulation in vacuoles and the conversion of sucrose to fructose by binding to and activating the promoters of the vacuolar sugar transporter MdTST2 and the neutral invertase MdNINV6. Additionally, MdNAC5 regulated the MdEIN3.4-MdSWEET15a module, strengthening the unloading of sucrose in the phloem of the fruit.
Our results reveal a new mechanism by which MdNAC5 regulates fructose accumulation in apples and provide theoretical foundations for improving apple sweetness through genetic modification.

查看原文本刊更多论文

MdNAC5：苹果果实中果糖积累的关键调节因子。

苹果果实的甜度是苹果品种改良的一个关键因素，而果糖是可溶性糖类中最甜的一种，对决定苹果的整体甜度起着至关重要的作用。因此，揭示控制果糖积累的关键基因和破译果糖的调控机制对苹果品种的改良至关重要。本研究通过对'长富2号'×'金美味'F1杂交群体进行BSA-seq和转录组分析，发现MdNAC5是果糖含量的关键调控基因。研究表明，MdNAC5 对苹果和番茄的果糖积累有显著影响。此外，我们还对苹果中的糖转运体和代谢酶进行了详细鉴定，发现 MdNAC5 可通过与液泡糖转运体 MdTST2 和中性转化酶 MdNINV6 的启动子结合并激活它们，从而增强液泡中的果糖积累以及蔗糖向果糖的转化。此外，MdNAC5 还调节了 MdEIN3.4-MdSWEET15a 模块，加强了果实韧皮部蔗糖的卸载。我们的研究结果揭示了 MdNAC5 调节苹果果糖积累的新机制，为通过基因改造提高苹果甜度提供了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.