Spatio-temporal distribution and international context of bovine viral diarrhoea virus genetic diversity in France.

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES
Claire Lescoat, Delphine Perrotte, Séverine Barry, Élise Oden, Valentin Herbet, Gaël Beaunée, Marc Tabouret, Fabienne Benoit, Pierre-Hugues Pitel, Véronique Duquesne, Xavier Bailly, Julien Thézé, Guy Kouokam
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引用次数: 0

Abstract

Bovine viral diarrhoea (BVD) is one of the most economically damaging livestock enzootic diseases in the world. BVD aetiological agents are three pestiviruses (BVDV-1, -2 and HoBi-like pestivirus), which exhibit high genetic diversity and complex transmission cycles. This considerably hampers the management of the disease, which is why eradication plans have been implemented in several countries. In France, a national plan has been in place since 2019. Our understanding of its impact on the distribution of BVDV genotypes is limited by the availability of French genetic data. Here, we conducted a molecular epidemiology study to refine our knowledge of BVDV genetic diversity in France, characterise its international relationships, and analyse national spatio-temporal genotypic distribution. We collated 1037 BVDV-positive samples throughout France between 2011 and 2023, with a greater sampling effort in two major cattle production areas. We developed a high-throughput sequencing protocol which we used to complete the 5'UTR genotyping of this collection. We show that two main BVDV-1 genotypes, 1e and 1b, account for 88% of genotyped sequences. We also identified seven other BVDV-1 genotypes occurring at low frequencies and three BVDV-2 samples (genotype 2c). Phylogenetic analyses indicate different worldwide distribution patterns between the two main BVDV-1 genotypes. Their relative frequencies present no major changes in France since the 1990s and few variations at the national scale. We also found some degree of local spatial structuring in western France. Overall, our results demonstrate the potential of large-scale sequence-based surveillance to monitor changes in the epidemiological situation of enzootic diseases.

法国牛病毒性腹泻病毒基因多样性的时空分布和国际背景。
牛病毒性腹泻(BVD)是世界上经济损失最大的牲畜流行病之一。BVD 的病原体是三种虫媒病毒(BVDV-1、-2 和 HoBi-like 虫媒病毒),它们表现出高度的遗传多样性和复杂的传播周期。这在很大程度上阻碍了对该疾病的管理,因此一些国家实施了根除计划。在法国,一项国家计划已于 2019 年开始实施。由于无法获得法国的基因数据,我们对该计划对 BVDV 基因型分布的影响的了解十分有限。在此,我们开展了一项分子流行病学研究,以完善我们对法国 BVDV 遗传多样性的了解,描述其国际关系,并分析国家时空基因型分布。我们整理了2011年至2023年期间全法国的1037份BVDV阳性样本,在两个主要牛产区加大了采样力度。我们开发了一种高通量测序方案,用于完成该样本的 5'UTR 基因分型。我们发现,1e 和 1b 这两种主要的 BVDV-1 基因型占基因分型序列的 88%。我们还发现了其他 7 个低频率出现的 BVDV-1 基因型和 3 个 BVDV-2 样本(基因型 2c)。系统发育分析表明,两种主要的 BVDV-1 基因型在全球的分布模式不同。自 20 世纪 90 年代以来,它们在法国的相对频率没有发生重大变化,在全国范围内也几乎没有变化。我们还在法国西部发现了一定程度的局部空间结构。总之,我们的研究结果证明了基于序列的大规模监测在监控流行病学状况变化方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
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