Lineage and sublineage analysis of human papillomavirus type 58 in iranian women.

IF 4 3区医学 Q2 VIROLOGY

Virology Journal Pub Date : 2024-10-03 DOI:10.1186/s12985-024-02521-9

Mohammad Shabanpour, Parvin Jalali-Alhosseini, Zabihollah Shoja, Fatemeh Ghafoori-Ghahdarijani, Sima Taherkhani, Somayeh Jalilvand

{"title":"Lineage and sublineage analysis of human papillomavirus type 58 in iranian women.","authors":"Mohammad Shabanpour, Parvin Jalali-Alhosseini, Zabihollah Shoja, Fatemeh Ghafoori-Ghahdarijani, Sima Taherkhani, Somayeh Jalilvand","doi":"10.1186/s12985-024-02521-9","DOIUrl":null,"url":null,"abstract":"Background: Variant analysis of distinct HPV types is important from different aspects including epidemiology, pathogenicity, and evolution.Methods: For this reason, the full sequence of the E6 and E7 genes of HPV 58 was examined in 130 HPV 58-infected cervical samples using PCR and sequencing.Results: Our results revealed that three lineages A, B, and D were found in this study; among which the B lineage was more common (91.50%). About sublineages, all samples of the B lineage belonged to the B1 sublineage, and samples that were classified as the A and D lineages were found to belong to the A1 (0.77%), A2 (5.38%), A3 (1.50%), and D2 (0.77%) sublineages. No statistically significant differences were found between lineages and stages of disease or amino acid changes (P > 0.05).Conclusion: Our results showed that lineage B, sublineage B1, was dominant in Iran. However, more studies with larger sample sizes from different parts of Iran are essential for assessing the pathogenicity risk of HPV 58 lineages in Iranian women with cervical cancer.","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"21 1","pages":"244"},"PeriodicalIF":4.0000,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11451209/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12985-024-02521-9","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VIROLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Variant analysis of distinct HPV types is important from different aspects including epidemiology, pathogenicity, and evolution.

Methods: For this reason, the full sequence of the E6 and E7 genes of HPV 58 was examined in 130 HPV 58-infected cervical samples using PCR and sequencing.

Results: Our results revealed that three lineages A, B, and D were found in this study; among which the B lineage was more common (91.50%). About sublineages, all samples of the B lineage belonged to the B1 sublineage, and samples that were classified as the A and D lineages were found to belong to the A1 (0.77%), A2 (5.38%), A3 (1.50%), and D2 (0.77%) sublineages. No statistically significant differences were found between lineages and stages of disease or amino acid changes (P > 0.05).

Conclusion: Our results showed that lineage B, sublineage B1, was dominant in Iran. However, more studies with larger sample sizes from different parts of Iran are essential for assessing the pathogenicity risk of HPV 58 lineages in Iranian women with cervical cancer.

查看原文本刊更多论文

伊朗妇女中人类乳头瘤病毒 58 型的系谱和亚系谱分析。

背景：从流行病学、致病性和进化等不同方面对不同类型的 HPV 进行变异分析非常重要：为此，我们利用 PCR 和测序技术对 130 例感染 HPV 58 的宫颈样本进行了 HPV 58 E6 和 E7 基因全序列检测：结果：我们的研究结果显示，本研究中发现了 A、B 和 D 三个系；其中 B 系更为常见（91.50%）。关于亚系，B 系的所有样本都属于 B1 亚系，被归类为 A 和 D 系的样本分别属于 A1（0.77%）、A2（5.38%）、A3（1.50%）和 D2（0.77%）亚系。各系与疾病阶段或氨基酸变化之间无统计学差异（P > 0.05）：我们的研究结果表明，B系（B1亚系）在伊朗占主导地位。然而，要评估伊朗妇女宫颈癌患者中 HPV 58 系的致病风险，必须在伊朗不同地区开展更多样本量更大的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.