Enhanced lymphangiogenesis in the left lateral segment of a biopsied liver during portoenterostomy for biliary atresia.

IF 1.5 3区医学 Q2 PEDIATRICS

Pediatric Surgery International Pub Date : 2024-10-04 DOI:10.1007/s00383-024-05845-3

Yudai Tsuruno, Koshiro Sugita, Toshihiro Muraji, Ryuta Masuya, Toshio Harumatsu, Keisuke Yano, Shun Onishi, Takafumi Kawano, Chihiro Ichikawa, Haruo Ohtani, Yuko Bitoh, Satoshi Ieiri

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引用次数: 0

Abstract

Purpose: We investigate the histopathology of the portal vein branches and lymphatic vessels to elucidate the mechanism of atrophy of the left lateral segment (LLS) of the liver in biliary atresia (BA).

Methods: LLS and right anterior segment (RAS) liver biopsy samples obtained during Kasai portoenterostomy (KPE) from ten consecutive patients with BA underwent histopathological investigation of the portal vein and lymphatic vessels using double chromogenic immunostaining for CD31/D2-40 and the hepatitis-like findings (HLF) score. Each parameter and clinical data were compared between prognostic groups.

Results: HLF scores in the LLS were always higher than those in the RAS. There was no difference in portal vein and lymphatic vascular morphology, whereas the number of lymphatic vessels was correlated with the fibrotic area of all specimen areas. Left-to-right ratio of the number of lymphatic vessels was correlated with the age at KPE (r = 0.784, p = 0.007) and the pre-KPE CRP value (r = 0.723, p = 0.018).

Conclusions: Lymphangiogenesis on the LLS compared to the RAS was significantly correlated with the degree of fibrosis and the age at KPE. Further investigation is warranted to clarify the causes of LLS atrophy and lymphangiogenesis relevant to immune dysregulation.

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在对胆道闭锁进行造口术时，活检肝脏左外侧部分的淋巴管生成增强。

目的：我们研究了门静脉分支和淋巴管的组织病理学，以阐明胆道闭锁（BA）患者肝脏左外侧段（LLS）萎缩的机制：方法：对连续十例胆道闭锁患者在葛西肠管造口术（KPE）中获得的肝左外侧段（LLS）和肝右前段（RAS）活检样本进行门静脉和淋巴管组织病理学检查，使用CD31/D2-40双色原免疫染色法和肝炎样病变（HLF）评分。对预后组的各项参数和临床数据进行比较：结果：LLS 组的 HLF 评分始终高于 RAS 组。门静脉和淋巴管形态无差异，而淋巴管数量与所有标本区域的纤维化面积相关。淋巴管数量的左右比值与KPE时的年龄（r = 0.784，p = 0.007）和KPE前的CRP值（r = 0.723，p = 0.018）相关：结论：LLS淋巴管生成与RAS淋巴管生成相比，与纤维化程度和KPE年龄显著相关。有必要进行进一步研究，以明确与免疫调节失调相关的 LLS 萎缩和淋巴管生成的原因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pediatric Surgery International 医学-外科

CiteScore

3.00

自引率

5.60%

发文量

215

审稿时长

3-6 weeks

期刊介绍： Pediatric Surgery International is a journal devoted to the publication of new and important information from the entire spectrum of pediatric surgery. The major purpose of the journal is to promote postgraduate training and further education in the surgery of infants and children. The contents will include articles in clinical and experimental surgery, as well as related fields. One section of each issue is devoted to a special topic, with invited contributions from recognized authorities. Other sections will include: -Review articles- Original articles- Technical innovations- Letters to the editor