Dynamic alterations and ecological implications of rice rhizosphere bacterial communities induced by an insect-transmitted reovirus across space and time.

IF 13.8 1区 生物学 Q1 MICROBIOLOGY
Nan Wu, Wenchong Shi, Lu Zhang, Hui Wang, Wenwen Liu, Yingdang Ren, Xiangdong Li, Zheng Gao, Xifeng Wang
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引用次数: 0

Abstract

Background: Cereal diseases caused by insect-transmitted viruses are challenging to forecast and control because of their intermittent outbreak patterns, which are usually attributed to increased population densities of vector insects due to cereal crop rotations and indiscriminate use of pesticides, and lack of resistance in commercial varieties. Root microbiomes are known to significantly affect plant health, but there are significant knowledge gaps concerning epidemics of cereal virus diseases at the microbiome-wide scale under a variety of environmental and biological factors.

Results: Here, we characterize the diversity and composition of rice (Oryza sativa) root-associated bacterial communities after infection by an insect-transmitted reovirus, rice black-streaked dwarf virus (RBSDV, genus Fijivirus, family Spinareoviridae), by sequencing the bacterial 16S rRNA gene amplified fragments from 1240 samples collected at a consecutive 3-year field experiment. The disease incidences gradually decreased from 2017 to 2019 in both Langfang (LF) and Kaifeng (KF). BRSDV infection significantly impacted the bacterial community in the rice rhizosphere, but this effect was highly susceptible to both the rice-intrinsic and external conditions. A greater correlation between the bacterial community in the rice rhizosphere and those in the root endosphere was found after virus infection, implying a potential relationship between the rice-intrinsic conditions and the rhizosphere bacterial community. The discrepant metabolites in rhizosphere soil were strongly and significantly correlated with the variation of rhizosphere bacterial communities. Glycerophosphates, amino acids, steroid esters, and triterpenoids were the metabolites most closely associated with the bacterial communities, and they mainly linked to the taxa of Proteobacteria, especially Rhodocyclaceae, Burkholderiaceae, and Xanthomonadales. In addition, the greenhouse pot experiments demonstrated that bulk soil microbiota significantly influenced the rhizosphere and endosphere communities and also regulated the RBSDV-mediated variation of rhizosphere bacterial communities.

Conclusions: Overall, this study reveals unprecedented spatiotemporal dynamics in rhizosphere bacterial communities triggered by RBSDV infection with potential implications for disease intermittent outbreaks. The finding has promising implications for future studies exploring virus-mediated plant-microbiome interactions. Video Abstract.

昆虫传播再病毒诱导的水稻根瘤菌群落跨时空的动态变化及其生态影响
背景:由昆虫传播的病毒引起的谷物疾病在预测和控制方面具有挑战性,因为其爆发模式时断时续,这通常归因于谷物轮作和滥用杀虫剂导致媒介昆虫的种群密度增加,以及商业品种缺乏抗性。众所周知,根部微生物组对植物健康有重大影响,但在各种环境和生物因素作用下,整个微生物组范围内谷物病毒病流行的相关知识还存在很大差距:结果:在此,我们通过对连续 3 年田间试验中采集的 1240 个样本的细菌 16S rRNA 基因扩增片段进行测序,描述了水稻(Oryza sativa)根部相关细菌群落在受到昆虫传播的再病毒--水稻黑条矮缩病病毒(RBSDV,Fijivirus 属,Spinareoviridae 科)--感染后的多样性和组成特征。从2017年到2019年,廊坊(LF)和开封(KF)的发病率逐渐下降。BRSDV感染对水稻根圈细菌群落有明显影响,但这种影响极易受水稻内在和外在条件的影响。病毒感染后,水稻根圈细菌群落与根内圈细菌群落之间的相关性更大,这意味着水稻内在条件与根圈细菌群落之间存在潜在的关系。根圈土壤中不同的代谢物与根圈细菌群落的变化密切相关。甘油磷酸酯、氨基酸、甾体酯和三萜类化合物是与细菌群落关系最密切的代谢物,它们主要与蛋白细菌类群有关,特别是罗氏菌科、伯克霍尔德菌科和黄单胞菌科。此外,温室盆栽实验表明,大体积土壤微生物群对根圈和内圈群落有显著影响,也调控了 RBSDV 介导的根圈细菌群落变化:总之,这项研究揭示了 RBSDV 感染引发的根圈细菌群落前所未有的时空动态变化,对疾病的间歇性爆发具有潜在影响。这一发现对今后探索病毒介导的植物微生物组相互作用的研究具有重要意义。视频摘要。
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来源期刊
Microbiome
Microbiome MICROBIOLOGY-
CiteScore
21.90
自引率
2.60%
发文量
198
审稿时长
4 weeks
期刊介绍: Microbiome is a journal that focuses on studies of microbiomes in humans, animals, plants, and the environment. It covers both natural and manipulated microbiomes, such as those in agriculture. The journal is interested in research that uses meta-omics approaches or novel bioinformatics tools and emphasizes the community/host interaction and structure-function relationship within the microbiome. Studies that go beyond descriptive omics surveys and include experimental or theoretical approaches will be considered for publication. The journal also encourages research that establishes cause and effect relationships and supports proposed microbiome functions. However, studies of individual microbial isolates/species without exploring their impact on the host or the complex microbiome structures and functions will not be considered for publication. Microbiome is indexed in BIOSIS, Current Contents, DOAJ, Embase, MEDLINE, PubMed, PubMed Central, and Science Citations Index Expanded.
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