Prevalence and molecular characteristics of carbapenem-resistant Escherichia coli isolated from dogs in South Korea.

IF 1.5 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Veterinary Science Pub Date : 2024-09-01 DOI:10.4142/jvs.24164

Bo-Youn Moon, Md Sekendar Ali, Seunghoe Kim, Hee-Seung Kang, Ye-Ji Kang, Jae-Myung Kim, Dong-Chan Moon, Suk-Kyung Lim

{"title":"Prevalence and molecular characteristics of carbapenem-resistant Escherichia coli isolated from dogs in South Korea.","authors":"Bo-Youn Moon, Md Sekendar Ali, Seunghoe Kim, Hee-Seung Kang, Ye-Ji Kang, Jae-Myung Kim, Dong-Chan Moon, Suk-Kyung Lim","doi":"10.4142/jvs.24164","DOIUrl":null,"url":null,"abstract":"Importance: Carbapenem-resistant Enterobacteriaceae are emerging as a global public health risk. Therefore, assessing the prevalence of carbapenem-resistant Escherichia coli (CRE) in both humans and animals is important.Objective: We aimed to ascertain the occurrence and characteristics of CRE isolated from companion animals, dogs and cats.Methods: E. coli strains were tested for antimicrobial susceptibility using the broth microdilution technique. Antimicrobial resistance genes were detected by polymerase chain reaction and sequencing analysis. The molecular characteristics of CRE were determined using multi-locus sequence typing, replicon typing, and pulsed-field gel electrophoresis (PFGE).Results: In total, 13 CRE isolates (0.13%) were identified from dogs possessing blaNDM-5 along with β-lactamase genes, mostly blaCMY-2 (92.2%) and blaTEM-1 (53.8%). The commonly observed mutations were S83L and D87N in gyrA, S80I in parC, and S458A in parE. CRE carried non-beta-lactam resistance genes, with the majority being tet(B) (100%), sul (84.6%), and aac(3)-II (53.8%). Nine different PFGE patterns (P1-P9), IncX3-type plasmids (69.2%), and ST410 (84.6%) were predominantly detected.Conclusions and relevance: This investigation provides significant insight into the prevalence and molecular characteristics of blaNDM-5-carrying E. coli in dogs. The co-existence of blaNDM-5 and other antimicrobial resistance genes in E. coli potentially poses severe health hazards to humans.","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 5","pages":"e67"},"PeriodicalIF":1.5000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450394/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.4142/jvs.24164","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Importance: Carbapenem-resistant Enterobacteriaceae are emerging as a global public health risk. Therefore, assessing the prevalence of carbapenem-resistant Escherichia coli (CRE) in both humans and animals is important.

Objective: We aimed to ascertain the occurrence and characteristics of CRE isolated from companion animals, dogs and cats.

Methods: E. coli strains were tested for antimicrobial susceptibility using the broth microdilution technique. Antimicrobial resistance genes were detected by polymerase chain reaction and sequencing analysis. The molecular characteristics of CRE were determined using multi-locus sequence typing, replicon typing, and pulsed-field gel electrophoresis (PFGE).

Results: In total, 13 CRE isolates (0.13%) were identified from dogs possessing bla_NDM-5 along with β-lactamase genes, mostly bla_CMY-2 (92.2%) and bla_TEM-1 (53.8%). The commonly observed mutations were S83L and D87N in gyrA, S80I in parC, and S458A in parE. CRE carried non-beta-lactam resistance genes, with the majority being tet(B) (100%), sul (84.6%), and aac(3)-II (53.8%). Nine different PFGE patterns (P1-P9), IncX3-type plasmids (69.2%), and ST410 (84.6%) were predominantly detected.

Conclusions and relevance: This investigation provides significant insight into the prevalence and molecular characteristics of bla_NDM-5-carrying E. coli in dogs. The co-existence of bla_NDM-5 and other antimicrobial resistance genes in E. coli potentially poses severe health hazards to humans.

查看原文本刊更多论文

从韩国狗体内分离的耐碳青霉烯类大肠埃希菌的流行率和分子特征。

重要性：耐碳青霉烯类肠杆菌科细菌正在成为一种全球公共卫生风险。因此，评估耐碳青霉烯类大肠杆菌（CRE）在人类和动物中的流行情况非常重要：我们旨在确定从伴侣动物（狗和猫）中分离出的 CRE 的发生率和特征：方法：使用肉汤微稀释技术检测大肠杆菌菌株的抗菌药敏感性。通过聚合酶链反应和测序分析检测抗菌药耐药性基因。使用多焦点序列分型、复制子分型和脉冲场凝胶电泳（PFGE）确定 CRE 的分子特征：结果：从狗身上共鉴定出 13 个 CRE 分离物（0.13%），它们都带有 blaNDM-5 和 β-内酰胺酶基因，其中大部分是 blaCMY-2 （92.2%）和 blaTEM-1（53.8%）。常见的突变是 gyrA 中的 S83L 和 D87N、parC 中的 S80I 和 parE 中的 S458A。CRE带有非beta-内酰胺耐药基因，其中大多数是tet(B)（100%）、sul（84.6%）和aac(3)-II（53.8%）。主要检测到九种不同的 PFGE 模式（P1-P9）、IncX3 型质粒（69.2%）和 ST410（84.6%）：这项调查为了解狗体内携带 blaNDM-5 大肠杆菌的流行情况和分子特征提供了重要依据。大肠杆菌中同时存在 blaNDM-5 和其他抗菌药耐药性基因可能会对人类健康造成严重危害。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Veterinary Science 农林科学-兽医学

CiteScore

3.10

自引率

5.60%

发文量

审稿时长

1.3 months

期刊介绍： The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.