Putative MutS2 Homologs in Algae: More Goods in Shopping Bag?

IF 2.1 3区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mariia Berdieva, Vera Kalinina, Olga Palii, Sergei Skarlato
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引用次数: 0

Abstract

MutS2 proteins are presumably involved in either control of recombination or translation quality control in bacteria. MutS2 homologs have been found in plants and some algae; however, their actual diversity in eukaryotes remains unknown. We found putative MutS2 homologs in various species of photosynthetic eukaryotes and performed a detailed analysis of the revealed amino acid sequences. Three groups of homologs were distinguished depending on their domain composition: MutS2 homologs with full set of specific domains, MutS2-like sequences without endonuclease Smr domain, and MutS2-like homologs lacking Smr and clamp in domain IV, the extreme form of which are proteins with only a complete ATPase domain. We clarified the information about amino acid composition and set of specific motifs in the conserved domains in MutS2 and MutS2-like sequences. The models of the predicted tertiary structure were obtained for each group of homologs. The phylogenetic analysis demonstrated that all eukaryotic sequences split into two large groups. The first group included homologs belonging to species of Archaeplastida and a subset of haptophyte homologs, while the second-sequences of organisms from CASH groups (cryptophytes, alveolates, stramenopiles, haptophytes) and chlorarachniophytes. The cyanobacterial MutS2 clustered together with the first group, and proteins belonging to Deltaproteobacteria (orders Myxococcales and Bradymonadales) showed phylogenetic affinity to the CASH-including group with strong support. The observed tree pattern did not support a clear differentiation of eukaryotes into lineages with red and green algae-derived plastids. The results are discussed in the context of current conceptions of serial endosymbioses and genetic mosaicism in algae with complex plastids.

藻类中的推定 MutS2 同源物:购物袋里还有更多商品?
在细菌中,MutS2 蛋白可能参与控制重组或翻译质量控制。在植物和一些藻类中也发现了 MutS2 同源物,但它们在真核生物中的实际多样性仍然未知。我们在各种光合真核生物中发现了推定的 MutS2 同源物,并对所揭示的氨基酸序列进行了详细分析。根据其结构域组成,我们将同源物分为三类:具有全套特异结构域的 MutS2 同源物、不含内切酶 Smr 结构域的 MutS2 类序列,以及结构域 IV 中缺少 Smr 和钳位的 MutS2 类同源物,其中的极端形式是仅具有完整 ATPase 结构域的蛋白质。我们澄清了MutS2和MutS2-like序列中保守结构域的氨基酸组成和特定基序的信息。我们获得了每组同源物的三级结构预测模型。系统进化分析表明,所有真核序列分为两大类。第一组包括属于弓形虫的同源物和一个七鳃鳗同源物子集,而第二组序列则包括来自 CASH 组(隐球藻、肺泡藻、葡萄孢藻、七鳃鳗)和绿藻的生物。蓝藻的 MutS2 与第一组聚类在一起,而属于 Deltaproteobacteria(Myxococcales 和 Bradymonadales 目)的蛋白质与包括 CASH 组的蛋白质在系统发育上有很强的亲缘关系。所观察到的树模式并不支持真核生物明显分化为具有红藻和绿藻质体的品系。本研究结合目前对具有复杂质体的藻类的序列内共生和遗传镶嵌的看法,对上述结果进行了讨论。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Molecular Evolution
Journal of Molecular Evolution 生物-进化生物学
CiteScore
5.50
自引率
2.60%
发文量
36
审稿时长
3 months
期刊介绍: Journal of Molecular Evolution covers experimental, computational, and theoretical work aimed at deciphering features of molecular evolution and the processes bearing on these features, from the initial formation of macromolecular systems through their evolution at the molecular level, the co-evolution of their functions in cellular and organismal systems, and their influence on organismal adaptation, speciation, and ecology. Topics addressed include the evolution of informational macromolecules and their relation to more complex levels of biological organization, including populations and taxa, as well as the molecular basis for the evolution of ecological interactions of species and the use of molecular data to infer fundamental processes in evolutionary ecology. This coverage accommodates such subfields as new genome sequences, comparative structural and functional genomics, population genetics, the molecular evolution of development, the evolution of gene regulation and gene interaction networks, and in vitro evolution of DNA and RNA, molecular evolutionary ecology, and the development of methods and theory that enable molecular evolutionary inference, including but not limited to, phylogenetic methods.
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