Effects of human amniotic membrane on the angiogenesis and healing of ischemic wounds in a rat model.

IF 2.3 4区医学 Q3 ENGINEERING, BIOMEDICAL

Journal of Biomaterials Applications Pub Date : 2024-10-04 DOI:10.1177/08853282241289919

Masato Sato, Kazuaki Tokodai, Kaoru Okada, Hiroyuki Ogasawara, Miyako Tanaka, Tetsuro Hoshiai, Masatoshi Saito, Hirofumi Sugawara, Daijirou Akamatsu, Michiaki Unno, Masafumi Goto, Takashi Kamei

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引用次数: 0

Abstract

Although the human amniotic membrane (hAM) has been demonstrated to promote angiogenesis, its efficacy in healing ischemic wounds remains unknown. Therefore, the current study aimed to evaluate the potential of hAM as a dressing for treating ischemic wounds. The inferior abdominal wall arteries and veins of male rats were divided, and an ischemic wound was created on each side of the abdominal wall. Of the two ischemic wounds created, only one was covered with hAM, and its wound healing effect was determined by measuring the wound area. Angiogenesis was assessed by measuring microvessel density (MVD). On day 5, the mean wound area changed from 400 mm² to 335.4 (260-450) mm² in the hAM group and to 459 (306-570) mm² in the control group (p = 0.0051). MVD was 19.0 (10.4-24.6) in the hAM group and 15.1 (10.6-20.8) in the control group (p = 0.0026). No significant differences in local pro- and anti-inflammatory cytokine levels were observed between the two groups. Histological examination revealed no rejection of the transplanted hAM. Therefore, the hAM may serve as a novel wound dressing that can promote angiogenesis and healing in ischemic wounds.

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人羊膜对大鼠模型缺血伤口血管生成和愈合的影响。

虽然人羊膜（hAM）已被证实可促进血管生成，但其在缺血性伤口愈合方面的功效仍不为人所知。因此，本研究旨在评估人羊膜作为敷料治疗缺血性伤口的潜力。研究人员分割了雄性大鼠的腹壁下动脉和静脉，并在腹壁两侧各造成一个缺血伤口。在两个缺血伤口中，只有一个伤口覆盖了 hAM，并通过测量伤口面积确定其伤口愈合效果。血管生成通过测量微血管密度（MVD）进行评估。第 5 天，hAM 组的平均伤口面积从 400 平方毫米变为 335.4（260-450）平方毫米，对照组则变为 459（306-570）平方毫米（p = 0.0051）。hAM 组的 MVD 为 19.0（10.4-24.6），对照组为 15.1（10.6-20.8）（p = 0.0026）。两组的局部促炎和抗炎细胞因子水平没有明显差异。组织学检查显示，移植的 hAM 没有排斥反应。因此，hAM 可作为一种新型伤口敷料，促进缺血性伤口的血管生成和愈合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biomaterials Applications 工程技术-材料科学：生物材料

CiteScore

5.10

自引率

3.40%

发文量

144

审稿时长

1.5 months

期刊介绍： The Journal of Biomaterials Applications is a fully peer reviewed international journal that publishes original research and review articles that emphasize the development, manufacture and clinical applications of biomaterials. Peer-reviewed articles by biomedical specialists from around the world cover: New developments in biomaterials, R&D, properties and performance, evaluation and applications Applications in biomedical materials and devices - from sutures and wound dressings to biosensors and cardiovascular devices Current findings in biological compatibility/incompatibility of biomaterials The Journal of Biomaterials Applications publishes original articles that emphasize the development, manufacture and clinical applications of biomaterials. Biomaterials continue to be one of the most rapidly growing areas of research in plastics today and certainly one of the biggest technical challenges, since biomaterial performance is dependent on polymer compatibility with the aggressive biological environment. The Journal cuts across disciplines and focuses on medical research and topics that present the broadest view of practical applications of biomaterials in actual clinical use. The Journal of Biomaterial Applications is devoted to new and emerging biomaterials technologies, particularly focusing on the many applications which are under development at industrial biomedical and polymer research facilities, as well as the ongoing activities in academic, medical and applied clinical uses of devices.