Extract from Falcaria vulgaris loaded with exosomes for the treatment of hypertension in pregnant mice: In vitro and In vivo investigations.

IF 1 4区医学 Q4 ENGINEERING, BIOMEDICAL

Bio-medical materials and engineering Pub Date : 2024-09-30 DOI:10.3233/BME-240053

Jing Chen, Huan Wang, Jing Zhu

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引用次数: 0

Abstract

Background: Hypertensive disorders during pregnancy pose significant risks to both maternal and fetal health, necessitating safe and effective therapeutic interventions.

Objective: This study aimed to investigate the potential of an extract derived from Falcaria vulgaris (FV), loaded with exosomes to form the Exo/FV complex, as a novel therapeutic agent for the management of hypertension in pregnant mice: antioxidants, antimicrobials, and phenolic compounds present in FV lower blood pressure.

Methods: The isolation of exosomes was done by ultracentrifugation methods and the FV was loaded into the exosomes by electroporation method.

Results: The Exo/FV was found to be spherical with diameter ranges from 20 to 30 nm and they were tested for biocompatibility in NHI 3T3 cell lines and found to be effective. This research investigated in vivo hypertension in mice induced by L-NAME and treated with FV and Exo/FV and found that AChE and MAO determine mice's redox state tends to reduce blood pressure. Increased non-protein thiol (NP-SH) and decreased lipid peroxidation were also found, and PDE-5, ACE, Arginase, and MDA activity has also been tested.

Conclusion: This analysis showed that Exo/FV effectively treated hypertension during pregnancy.

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含有外泌体的法桐提取物用于治疗怀孕小鼠的高血压：体外和体内研究。

背景：妊娠期高血压疾病对母体和胎儿的健康构成重大风险，因此需要安全有效的治疗干预措施：本研究的目的是研究一种从蚕豆（Falcaria vulgaris，FV）中提取的提取物与外泌体形成 Exo/FV 复合物，作为一种新型治疗剂治疗妊娠小鼠高血压的潜力：FV 中的抗氧化剂、抗菌剂和酚类化合物可降低血压：方法：用超速离心法分离外泌体，用电穿孔法将 FV 加入外泌体：结果：外泌体/FV呈球形，直径在20至30纳米之间。这项研究调查了由 L-NAME 诱导并用 FV 和 Exo/FV 治疗的小鼠体内高血压情况，发现 AChE 和 MAO 决定小鼠的氧化还原状态，并倾向于降低血压。此外，还发现了非蛋白质硫醇（NP-SH）的增加和脂质过氧化的减少，并检测了 PDE-5、ACE、精氨酸酶和 MDA 的活性：分析表明，Exo/FV 能有效治疗妊娠期高血压。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bio-medical materials and engineering 工程技术-材料科学：生物材料

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.