Molecular identification of a putative novel varicosavirus in Agastache rugosa in China

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2024-10-04 DOI:10.1007/s00705-024-06141-0

Caixia Yang, Lei Yang, Yujie Li, Xiaoling Cui, Xue Bai, Song Zhang, Mengji Cao

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Abstract

A putative novel virus was identified in Agastache rugosa in China and tentatively named "Agastache rugosa-associated varicosavirus" (ARaVV). The nearly complete genome sequence of ARaVV was obtained through RNA sequencing (RNA-seq) and subsequent RT-PCR. The ARaVV genome consists of two negative-sense single-stranded RNA segments that are 6428 and 3862 nucleotides (nt) in size, respectively. RNA1 encodes a large polymerase (L), and RNA2 encodes a putative nucleocapsid protein (N), protein 2 (P2), and protein 3 (P3). The L protein shared the highest amino acid (aa) sequence identity (51.3%) with Erysimum virus 1 (EryV1, BK061766.1). The N, P2, and P3 shared the highest aa sequence identity (33.1%, 14.0%, and 24.2%) with Leucanthemum virus 1, Raphanus virus 1, and Spinach virus 1, respectively. Phylogenetic analysis based on amino acid sequences of the L protein showed that ARaVV clustered in a clade with the varicosaviruses, indicating that ARaVV is a putative new member of the genus Varicosavirus.

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中国姬松茸中一种推定的新型变异病毒的分子鉴定。

在中国姬松茸（Agastache rugosa）中发现了一种推测的新型病毒，并初步命名为 "姬松茸相关变异病毒"（Agastache rugosa-associated varicosavirus，ARaVV）。通过RNA测序（RNA-seq）和随后的RT-PCR，获得了ARaVV近乎完整的基因组序列。ARaVV 基因组由两个负义单链 RNA 片段组成，大小分别为 6428 和 3862 个核苷酸（nt）。RNA1 编码大型聚合酶（L），RNA2 编码假定核壳蛋白（N）、蛋白 2（P2）和蛋白 3（P3）。L 蛋白与 Erysimum virus 1（EryV1，BK061766.1）的氨基酸序列相同度最高（51.3%）。N、P2 和 P3 与韭菜病毒 1、油菜病毒 1 和菠菜病毒 1 的氨基酸序列相同度最高（分别为 33.1%、14.0% 和 24.2%）。基于 L 蛋白氨基酸序列的系统进化分析表明，ARaVV 与变异病毒聚为一个支系，表明 ARaVV 是变异病毒属的一个推定新成员。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.