Predominance of enterotoxigenic Escherichia coli among ESBL/plasmid-mediated AmpC-producing strains isolated from diarrheic foals: a public health concern.

IF 1.9 2区 农林科学 Q2 VETERINARY SCIENCES
Ahmed Samir, Khaled A Abdel-Moein, Hala M Zaher
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引用次数: 0

Abstract

Background: The upsurge of diarrheagenic E. coli pathotypes carrying extended-spectrum beta-lactamases (ESBLs)/plasmid-mediated AmpC β-lactamase (pAmpC) among animals constitutes an emerging threat for humans and animals. This study investigated the burden of ESBL-/pAmpC-producing diarrheagenic E. coli among diarrheic foals and its potential public health implications. Rectal swabs were collected from 80 diarrheic foals. These swabs were processed to isolate and identify ESBL/pAmpC-producing E. coli using a selective culture medium, biochemical tests, phenotypic identification, and molecular identification of ESBL- and pAmpC-encoding genes. Moreover, all ESBL-/pAmpC-producing E. coli isolates were examined for different virulence genes related to diarrheagenic E. coli pathotypes.

Results: Out of 80 examined foals, 26 (32.5%) were confirmed as ESBL-/pAmpC-producing E. coli, of which 14 (17.5%) animals carried only ESBL-producing E. coli, whereas 12 (15%) animals possessed ESBL-pAmpC-producing E. coli. The only detected diarrheagenic pathotype was enterotoxigenic, encoded by the heat-stable enterotoxin gene (ST) with a prevalence rate of 80.8% (21/26). The ST gene was further characterized where STa, STb, and STa + STb were found in one, four, and 16 strains, respectively. Moreover, all enterotoxigenic E. coli (ETEC) isolates exhibited a multidrug-resistance pattern. The phylogenetic analysis of 3 obtained partial STb sequences revealed high genetic relatedness to ETEC isolates retrieved from humans, conferring such sequences' public health significance.

Conclusions: These findings highlight that diarrheic foals could serve as a potential reservoir for multidrug-resistant ESBL-/pAmpC-producing enterotoxigenic E. coli.

从腹泻马驹中分离出的产ESBL/质粒介导的AmpC菌株中,肠毒性大肠埃希菌占主导地位:公共卫生问题。
背景:动物中携带广谱β-内酰胺酶(ESBLs)/质粒介导的AmpC β-内酰胺酶(pAmpC)的腹泻性大肠杆菌病型激增,对人类和动物构成了新的威胁。本研究调查了腹泻马驹中产生 ESBL-/pAmpC 的腹泻性大肠杆菌的负担及其对公共卫生的潜在影响。研究人员采集了 80 只腹泻马驹的直肠拭子。对这些拭子进行处理,利用选择性培养基、生化测试、表型鉴定以及 ESBL 和 pAmpC 编码基因的分子鉴定来分离和鉴定产 ESBL/pAmpC 大肠杆菌。此外,还对所有产ESBL-/pAmpC大肠杆菌分离物进行了与致腹泻大肠杆菌病型相关的不同毒力基因检测:结果:在80只接受检查的马驹中,26只(32.5%)被确认为产ESBL-/pAmpC大肠杆菌,其中14只(17.5%)仅携带产ESBL大肠杆菌,而12只(15%)携带产ESBL-pAmpC大肠杆菌。唯一检测到的腹泻致病型是肠毒性,由热稳定肠毒素基因(ST)编码,发病率为 80.8%(21/26)。对 ST 基因进行了进一步鉴定,发现 STa、STb 和 STa + STb 分别存在于 1 株、4 株和 16 株菌株中。此外,所有肠毒性大肠杆菌(ETEC)分离株都表现出多重耐药模式。对获得的 3 个 STb 部分序列进行的系统发育分析表明,这些序列与从人类身上分离出的 ETEC 具有高度的遗传亲缘关系,因此具有公共卫生意义:这些研究结果表明,腹泻马驹可能是耐多药 ESBL-/pAmpC 产肠毒性大肠杆菌的潜在储库。
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来源期刊
Acta Veterinaria Scandinavica
Acta Veterinaria Scandinavica 农林科学-兽医学
CiteScore
3.60
自引率
0.00%
发文量
28
审稿时长
18-36 weeks
期刊介绍: Acta Veterinaria Scandinavica is an open access journal encompassing all aspects of veterinary research and medicine of domestic and wild animals.
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