Reading m6A marks in mRNA: A potent mechanism of gene regulation in plants

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Thi Kim Hang Nguyen, Hunseung Kang
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引用次数: 0

Abstract

Modifications to RNA have recently been recognized as a pivotal regulator of gene expression in living organisms. More than 170 chemical modifications have been identified in RNAs, with N6-methyladenosine (m6A) being the most abundant modification in eukaryotic mRNAs. The addition and removal of m6A marks are catalyzed by methyltransferases (referred to as “writers”) and demethylases (referred to as “erasers”), respectively. In addition, the m6A marks in mRNAs are recognized and interpreted by m6A-binding proteins (referred to as “readers”), which regulate the fate of mRNAs, including stability, splicing, transport, and translation. Therefore, exploring the mechanism underlying the m6A reader-mediated modulation of RNA metabolism is essential for a much deeper understanding of the epigenetic role of RNA modification in plants. Recent discoveries have improved our understanding of the functions of m6A readers in plant growth and development, stress response, and disease resistance. This review highlights the latest developments in m6A reader research, emphasizing the diverse RNA-binding domains crucial for m6A reader function and the biological and cellular roles of m6A readers in the plant response to developmental and environmental signals. Moreover, we propose and discuss the potential future research directions and challenges in identifying novel m6A readers and elucidating the cellular and mechanistic role of m6A readers in plants.

Abstract Image

读取 mRNA 中的 m6A 标记:植物基因调控的有效机制
最近,人们认识到 RNA 的修饰是生物体内基因表达的关键调节因子。目前已在 RNA 中发现 170 多种化学修饰,其中 N6-甲基腺苷(m6A)是真核生物 mRNA 中最常见的修饰。m6A 标记的添加和去除分别由甲基转移酶(被称为 "写手")和去甲基化酶(被称为 "擦手")催化。此外,mRNA 中的 m6A 标记会被 m6A 结合蛋白(称为 "阅读者")识别和解读,从而调控 mRNA 的命运,包括稳定性、剪接、运输和翻译。因此,探索 m6A 阅读器介导的 RNA 代谢调节机制对于更深入地了解 RNA 修饰在植物中的表观遗传作用至关重要。最近的发现增进了我们对 m6A 阅读器在植物生长发育、胁迫响应和抗病中的功能的了解。本综述重点介绍了 m6A 阅读器研究的最新进展,强调了对 m6A 阅读器功能至关重要的各种 RNA 结合域,以及 m6A 阅读器在植物对发育和环境信号的响应中的生物和细胞作用。此外,我们还提出并讨论了在鉴定新型 m6A 阅读器和阐明 m6A 阅读器在植物中的细胞和机理作用方面潜在的未来研究方向和挑战。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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