Metabolic activities of marine ammonia-oxidizing archaea orchestrated by quorum sensing.

IF 4.5 Q1 MICROBIOLOGY
mLife Pub Date : 2024-09-30 eCollection Date: 2024-09-01 DOI:10.1002/mlf2.12144
Olivier Pereira, Wei Qin, Pierre E Galand, Didier Debroas, Raphael Lami, Corentin Hochart, Yangkai Zhou, Jin Zhou, Chuanlun Zhang
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引用次数: 0

Abstract

Ammonia-oxidizing archaea (AOA) play crucial roles in marine carbon and nitrogen cycles by fixing inorganic carbon and performing the initial step of nitrification. Evaluation of carbon and nitrogen metabolism popularly relies on functional genes such as amoA and accA. Increasing studies suggest that quorum sensing (QS) mainly studied in biofilms for bacteria may serve as a universal communication and regulatory mechanism among prokaryotes; however, this has yet to be demonstrated in marine planktonic archaea. To bridge this knowledge gap, we employed a combination of metabolic activity markers (amoA, accA, and grs) to elucidate the regulation of AOA-mediated nitrogen, carbon processes, and their interactions with the surrounding heterotrophic population. Through co-transcription investigations linking metabolic markers to potential key QS genes, we discovered that QS molecules could regulate AOA's carbon, nitrogen, and lipid metabolisms under different conditions. Interestingly, specific AOA ecotypes showed a preference for employing distinct QS systems and a distinct QS circuit involving a typical population. Overall, our data demonstrate that QS orchestrates nitrogen and carbon metabolism, including the exchange of organic metabolites between AOA and surrounding heterotrophic bacteria, which has been previously overlooked in marine AOA research.

通过法定人数感应协调海洋氨氧化古菌的代谢活动。
氨氧化古细菌(AOA)通过固定无机碳和执行硝化的初始步骤,在海洋碳氮循环中发挥着至关重要的作用。对碳氮代谢的评估通常依赖于功能基因,如 amoA 和 accA。越来越多的研究表明,主要在细菌生物膜中研究的法定量感应(QS)可能是原核生物之间的一种普遍交流和调控机制;然而,这一点尚未在海洋浮游古细菌中得到证实。为了弥补这一知识空白,我们采用了代谢活性标记(amoA、accA 和 grs)组合来阐明 AOA 介导的氮、碳过程的调控及其与周围异养生物群体的相互作用。通过将代谢标记与潜在的关键 QS 基因进行共转录研究,我们发现 QS 分子可以在不同条件下调控 AOA 的碳、氮和脂代谢。有趣的是,特定的 AOA 生态型表现出偏好使用不同的 QS 系统和涉及典型种群的不同 QS 回路。总之,我们的数据证明了 QS 能够协调氮和碳的代谢,包括 AOA 与周围异养菌之间有机代谢物的交换,而这在海洋 AOA 研究中一直被忽视。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
2.30
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