Rhodopseudomonas palustris Atp2 Protein Exerts Antifungal Effects by Targeting the Ribosomal Protein MoRpl12 in Magnaporthe oryzae.

IF 2.6 2区农林科学 Q2 PLANT SCIENCES

Phytopathology Pub Date : 2024-10-01 DOI:10.1094/PHYTO-05-24-0169-R

Chunyan Chen, Xiyang Wu, Qiang Huang, Yingfei Qin, Chenggang Li, Xin Zhang, Pei Wang, Xinqiu Tan, Yong Liu, Yue Chen, Deyong Zhang

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引用次数: 0

Abstract

Rice blast is one of the most hazardous diseases affecting rice production. Previously, we discovered that the Atp2 protein of Rhodopseudomonas palustris could significantly inhibit the appressorium formation and pathogenicity of Magnaporthe oryzae. However, the molecular mechanism of this fungus has remained unknown. This study revealed that Atp2 can enter the cell and interact with the ribosomal protein MoRpl12 of M. oryzae, directly affecting the expression of the MoRpl12 protein. Silencing the MoRPL12 gene can affect cell wall integrity, growth, conidiogenesis, and fungal pathogenicity. The quantitative reverse transcription PCR results showed significant changes in the expression of conidiation-related genes in the MoRPL12 gene-silenced mutants or in the Atp2 protein-treated plants. We further found that Atp2 treatment can influence the expression of ribosomal-related genes, such as RPL, in M. oryzae. Our study revealed a novel antifungal mechanism by which the Atp2 protein binds to the ribosomal protein MoRpl12 and inhibits the pathogenicity of rice blast fungus, providing a new potential target for rice blast prevention and control.

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黄绿假单胞菌 Atp2 蛋白通过靶向 Magnaporthe oryzae 的核糖体蛋白 MoRpl12 发挥抗真菌作用

稻瘟病是影响水稻生产最严重的病害之一。此前，我们发现淡水红假单胞菌（Rhodopseudomonas palustris）的 Atp2 蛋白能显著抑制稻瘟病菌（Magnaporthe oryzae）的附着体形成和致病性。然而，这种真菌的分子机制一直不为人知。本研究发现，Atp2能进入细胞并与M. oryzae的核糖体蛋白MoRpl12相互作用，直接影响MoRpl12蛋白的表达。沉默 MoRPL12 基因可影响细胞壁的完整性、生长、分生孢子的发生和真菌的致病性。反转录定量 PCR 结果显示，MoRPL12 基因沉默突变体或经 Atp2 蛋白处理的植株中，分生相关基因的表达发生了显著变化。我们进一步发现，Atp2 处理可影响 M. oryzae 中核糖体相关基因（如 RPL）的表达。我们的研究揭示了 Atp2 蛋白与核糖体蛋白 MoRpl12 结合并抑制稻瘟病菌致病性的新型抗真菌机制，为稻瘟病防控提供了一个新的潜在靶标。

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来源期刊

Phytopathology 生物-植物科学

CiteScore

5.90

自引率

9.40%

发文量

505

审稿时长

4-8 weeks

期刊介绍： Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.