The rat bladder umbrella cell keratin network: Organization, dependence on the plectin cytolinker, and responses to bladder filling.

IF 3.1 3区 生物学 Q3 CELL BIOLOGY
Molecular Biology of the Cell Pub Date : 2024-11-01 Epub Date: 2024-10-02 DOI:10.1091/mbc.E24-06-0262
Wily G Ruiz, Dennis R Clayton, Tanmay Parakala-Jain, Marianela G Dalghi, Jonathan Franks, Gerard Apodaca
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Abstract

The keratin cytoskeleton and associated desmosomes contribute to the mechanical stability of epithelial tissues, but their organization in native bladder umbrella cells and their responses to bladder filling are poorly understood. Using whole rat bladders in conjunction with confocal microscopy, super-resolution image processing, three-dimensional image reconstruction, and platinum replica electron microscopy, we identified a cortical cytoskeleton network in umbrella cells that was organized as a dense tile-like mesh comprised of tesserae bordered by cortical actin filaments, filled with keratin filaments, and cross-linked by plectin. Below these tesserae, keratin formed a subapical meshwork and at the cell periphery a band of keratin was linked via plectin to the junction-associated actin ring. Disruption of plectin led to focal keratin network dissolution, loss of the junction-associated keratin, and defects in cell-cell adhesion. During bladder filling, a junction-localized necklace of desmosomes expanded, and a subjacent girded layer formed linking the keratin network to desmosomes, including those at the umbrella cell-intermediate cell interface. Our studies reveal a novel tile- and mesh-like organization of the umbrella cell keratin network that is dependent on plectin, that reorganizes in response to bladder filling, and that likely serves to maintain umbrella cell continuity in the face of mechanical distension.

大鼠膀胱伞细胞角蛋白网络:组织、对 plectin 细胞连接蛋白的依赖以及对膀胱充盈的反应。
角蛋白细胞骨架和相关的脱膜体有助于上皮组织的机械稳定性,但人们对它们在原生膀胱伞细胞中的组织结构及其对膀胱充盈的反应知之甚少。我们利用大鼠的整个膀胱,结合共聚焦显微镜、超分辨率图像处理、三维图像重建和铂复制电子显微镜,确定了伞状细胞中的皮质细胞骨架网络,该网络组织成密集的瓦片状网状结构,由以皮质肌动蛋白丝为边界的方格组成,充满角蛋白丝,并由角蛋白交联。在这些方格下面,角蛋白形成了一个近尖端的网状结构,在细胞外围,一条角蛋白带通过 plectin 与交界处的肌动蛋白环相连。干扰 plectin 会导致局灶性角蛋白网络解体、交界处相关角蛋白丢失以及细胞-细胞粘附缺陷。在膀胱充盈过程中,交界处定位的脱膜小体项链扩大,邻近的束带层形成,将角蛋白网络与脱膜小体(包括伞状细胞-中间细胞界面的脱膜小体)连接起来。我们的研究揭示了伞细胞角蛋白网络的新型瓦状和网状组织,这种组织依赖于plectin,会随着膀胱充盈而重组,很可能是为了在面对机械拉伸时保持伞细胞的连续性。媒体:见正文] [媒体:见正文] [媒体:见正文] [媒体:见正文] [媒体:见正文] [媒体:见正文] [媒体:见正文] [媒体:见正文]。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular Biology of the Cell
Molecular Biology of the Cell 生物-细胞生物学
CiteScore
6.00
自引率
6.10%
发文量
402
审稿时长
2 months
期刊介绍: MBoC publishes research articles that present conceptual advances of broad interest and significance within all areas of cell, molecular, and developmental biology. We welcome manuscripts that describe advances with applications across topics including but not limited to: cell growth and division; nuclear and cytoskeletal processes; membrane trafficking and autophagy; organelle biology; quantitative cell biology; physical cell biology and mechanobiology; cell signaling; stem cell biology and development; cancer biology; cellular immunology and microbial pathogenesis; cellular neurobiology; prokaryotic cell biology; and cell biology of disease.
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