The Parental Centromere Sizes Remain Unaltered in Allopolyploid Wheat-Rye Hybrids.

Abstract

Introduction: In chromatin nucleosomes, the presence - instead of canonical histone H3 - of its variant, CENH3 (in plants), is considered the most reliable marker of the location of centromeres. In this study, we investigated the effects of distant hybridization and maternal cytoplasm on centromere size in allopolyploid hybrids between wheat and rye as compared to their parental forms.

Methods: Centromere sizes were measured using 2D images of CENH3 fluorescent signals on interphase nuclei obtained from parental forms and a triticale hybrid (genomic formula AABBBRR), in which the maternal form is wheat and secalotriticum hybrids (genomic formula RRAABBB) in which the maternal form is rye. For measurements, we selected the largest spherical nuclei with large nucleoli in the late G2 phase, in which most of the loading of CENH3 into centromeric chromatin takes place.

Results: When processing the results of the measurement of centromere sizes in the hybrids, the obtained values were compared with those expected for the case of no change in centromere sizes in any of the parental sets of chromosomes. We found no significant differences between expected and measured values.

Conclusion: We believe that, in the case of allopolyploid hybrids between wheat and rye, centromeres of chromosomes from the parental species retain the sizes formed during evolution. This conservatism may be promoted by the high similarity in the structure of the CENH3 molecules between these species.