Titanium surfaces loaded with puerarin and exosomes derived from adipose stem cells promote the proliferation and differentiation of pre-osteoblasts.

IF 1.9 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Dental materials journal Pub Date : 2024-12-10 Epub Date: 2024-10-02 DOI:10.4012/dmj.2024-066

Yan Wang, Changlong Zhou, Qianqian Guo, Bin Chen, Jia Luo, Yimin Lv

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引用次数: 0

Abstract

The study is to evaluate the effects of collagen/hyaluronic acid coating with or without puerarin and exosomes (Exos) derived from adipose stem cells (ADSCs-Exos) on pre-osteoblast proliferation and differentiation on the surface of titanium materials. Titanium materials with different coatings were prepared by layer-by-layer technique, evaluating the surface characterization. Cell functions were assessed by cell biology experiments. Related genes and proteins were assessed by RT-qPCR and Western blot. Puerarin or ADSCs-Exos coating had better effects on promoting the adhesion, proliferation and differentiation of pre-osteoblasts, and the strongest effect was found after their co-coatings, manifesting as the up-regulations of alkaline phosphatase (ALP) activity, collagen type I alpha 1 (Col1a1), runt-related transcription factor 2 (Runx2), osterix and activating transcription factor-2 (ATF-2). Levels of phosphorylated-P38 (p-P38) and p-ATF-2 were up-regulated in pre-osteoblasts grown on puerarin and ADSCs-Exos-loaded titanium surfaces. Titanium surfaces loaded with puerarin and ADSCs-Exos promotes the proliferation and differentiation of pre-osteoblasts.

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加载了葛根素和脂肪干细胞外泌体的钛表面能促进前成骨细胞的增殖和分化。

本研究旨在评估添加或不添加葛根素的胶原蛋白/透明质酸涂层和脂肪干细胞衍生的外泌体（Exos）（ADSCs-Exos）对钛材料表面前成骨细胞增殖和分化的影响。通过逐层技术制备了具有不同涂层的钛材料，并对其表面特征进行了评估。细胞功能通过细胞生物学实验进行评估。相关基因和蛋白质通过 RT-qPCR 和 Western 印迹进行评估。Puerarin或ADSCs-Exos涂层对促进前成骨细胞的粘附、增殖和分化有更好的效果，它们共同涂层后的效果最强，表现为碱性磷酸酶（ALP）活性、Ⅰ型α1胶原（Col1a1）、Runt相关转录因子2（Runx2）、sterix和活化转录因子-2（ATF-2）的上调。在葛根素和 ADSCs-Exos 负载的钛表面生长的前成骨细胞中，磷酸化-P38（p-P38）和 p-ATF-2 的水平上调。负载葛根素和 ADSCs-Exos 的钛表面可促进前成骨细胞的增殖和分化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dental materials journal 医学-材料科学：生物材料

CiteScore

4.60

自引率

4.00%

发文量

102

审稿时长

3 months

期刊介绍： Dental Materials Journal is a peer review journal published by the Japanese Society for Dental Materials and Devises aiming to introduce the progress of the basic and applied sciences in dental materials and biomaterials. The dental materials-related clinical science and instrumental technologies are also within the scope of this journal. The materials dealt include synthetic polymers, ceramics, metals and tissue-derived biomaterials. Forefront dental materials and biomaterials used in developing filed, such as tissue engineering, bioengineering and artificial intelligence, are positively considered for the review as well. Recent acceptance rate of the submitted manuscript in the journal is around 30%.