Regulation of maize growth and immunity by ZmSKI3-mediated RNA decay and post-transcriptional gene silencing.

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jie Gao, Na Zhang, Guohui Liu, Jinjun Tian, Mengyao Chen, Ying Wang, Ye Xing, Ying Zhang, Chenyang Zhao, Xiaohuan Mu, Yanwen Yu, Hongbin Niu, Jiankun Li, Jihua Tang, Mingyue Gou
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引用次数: 0

Abstract

Disease resistance is often associated with compromised plant growth and yield due to defense-growth tradeoffs. However, key components and mechanisms underlying the defense-growth tradeoffs are rarely explored in maize. In this study, we find that ZmSKI3, a putative subunit of the SUPERKILLER (SKI) complex that mediates the 3'-5' degradation of RNA, regulates both plant development and disease resistance in maize. The Zmski3 mutants showed retarded plant growth and constitutively activated defense responses, while the ZmSKI3 overexpression lines are more susceptible to Curvularia lunata and Bipolaris maydis. Consistently, the expression of defense-related genes was generally up-regulated, while expressions of growth-related genes were mostly down-regulated in leaves of the Zmski3-1 mutant compared to that of wild type. In addition, 223 differentially expressed genes that are up-regulated in Zmski3-1 mutant but down-regulated in the ZmSKI3 overexpression line are identified as potential target genes of ZmSKI3. Moreover, small interfering RNAs targeting the transcripts of the defense- and growth-related genes are differentially accumulated, likely to combat the increase of defense-related transcripts but decrease of growth-related transcripts in Zmski3-1 mutant. Taken together, our study indicates that plant growth and immunity could be regulated by both ZmSKI3-mediated RNA decay and post-transcriptional gene silencing in maize.

ZmSKI3 介导的 RNA 衰变和转录后基因沉默对玉米生长和免疫的调控。
抗病性往往与防御-生长权衡导致的植物生长和产量受损有关。然而,在玉米中,很少有人探索防御-生长权衡的关键成分和机制。在这项研究中,我们发现 ZmSKI3 是超级杀手(SKI)复合体的一个推定亚基,它介导 RNA 的 3'-5' 降解,同时调控玉米的植物生长和抗病性。Zmski3 突变体表现出植物生长迟缓和组成性激活的防御反应,而 ZmSKI3 过表达株系则更易感染 Curvularia lunata 和 Bipolaris maydis。与野生型相比,Zmski3-1 突变体叶片中防御相关基因的表达普遍上调,而生长相关基因的表达大多下调。此外,在 Zmski3-1 突变体中上调而在 ZmSKI3 过表达株中下调的 223 个差异表达基因被鉴定为 ZmSKI3 的潜在靶基因。此外,针对防御和生长相关基因转录本的小干扰 RNA 有不同程度的积累,这可能是 Zmski3-1 突变体中防御相关转录本增加而生长相关转录本减少的原因。综上所述,我们的研究表明,玉米中的植物生长和免疫可同时受到 ZmSKI3 介导的 RNA 衰变和转录后基因沉默的调控。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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