Sha Li, Lei Zhang, Qiuyi Xu, Meiju Sui, Long Xiao, Daiqin Chen, Zhong-Xing Jiang, Xin Zhou, Shizhen Chen
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引用次数: 0
Abstract
Acute lung injury (ALI) is a severe complication in clinical settings. Alert diagnosis and severity assessment of ALI is pivotal to ensure curative treatment and increase survival rates. However, the development of a precise ALI diagnostic strategy remains a pending task. Here, leveraging neutrophil's inflammation-homing and physiological barrier-navigating capability, a facile strategy is proposed for achieving targeted 19F-MRI detection of ALI based on the nanoengineered neutrophil internalized with perfluorocarbon nanoemulsion (Neu@PFC). The remodeling process poses a negligible impact on the neutrophil's inherent activation and transmigration functions. The migratory behavior of Neu@PFC toward pneumonia is confirmed in vivo using an LPS-induced ALI murine model. Direct intratracheal (i.t.) administration contributes to a vast deposition of Neu@PFC within the lung, allowing for real-time 19F-MRI visualization and the potential to predict progressive pneumonia. Furthermore, intravenous (i.v.) administration of Neu@PFC enables quantitative assessment of the extent of ALI due to the chemokine-guided neutrophil migration. This study not only provides a pathway to diagnose ALI, but also sheds light on the neutrophil recruitment and activation cues in different tissues and inflammatory conditions, which is a prerequisite for developing potential therapeutic approaches.
急性肺损伤(ALI)是临床上的一种严重并发症。急性肺损伤的快速诊断和严重程度评估对于确保治疗效果和提高存活率至关重要。然而,制定精确的 ALI 诊断策略仍是一项有待完成的任务。本文利用中性粒细胞的炎症归巢和生理屏障导航能力,提出了一种基于全氟碳化物纳米乳液(Neu@PFC)内化的纳米工程中性粒细胞的简便策略,以实现 19F-MRI 对 ALI 的靶向检测。重塑过程对中性粒细胞固有的活化和迁移功能影响微乎其微。Neu@PFC 对肺炎的迁移行为是通过 LPS 诱导的 ALI 小鼠模型在体内证实的。气管内直接给药有助于 Neu@PFC 在肺内的大量沉积,从而实现 19F-MRI 实时可视化,并有可能预测进展性肺炎。此外,通过静脉注射 Neu@PFC 可以定量评估化疗因子引导的中性粒细胞迁移导致的 ALI 程度。这项研究不仅提供了诊断 ALI 的途径,还揭示了中性粒细胞在不同组织和炎症条件下的募集和激活线索,这是开发潜在治疗方法的先决条件。
期刊介绍:
Advanced Materials, one of the world's most prestigious journals and the foundation of the Advanced portfolio, is the home of choice for best-in-class materials science for more than 30 years. Following this fast-growing and interdisciplinary field, we are considering and publishing the most important discoveries on any and all materials from materials scientists, chemists, physicists, engineers as well as health and life scientists and bringing you the latest results and trends in modern materials-related research every week.