LAPONITE® nano-silicates potentiate the angiogenic effects of FG-4592 and osteogenic effects of BMP-2.

IF 5.8 3区医学 Q1 MATERIALS SCIENCE, BIOMATERIALS

Biomaterials Science Pub Date : 2024-10-03 DOI:10.1039/d4bm00636d

Bipin Gaihre, Emily Camilleri, Maryam Tilton, Maria D Astudillo Potes, Xifeng Liu, Fabrice Lucien, Lichun Lu

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引用次数: 0

Abstract

LAPONITE®-based drug delivery systems offer many advantages due to the unique ionic and physical properties of LAPONITE®. The high ionicity and large surface area of LAPONITE® nanoparticles enable the intercalation and dissolution of biomolecules. In this study, we explored the potential of LAPONITE® as a carrier for FG-4592 to support angiogenesis and as a carrier for bone morphogenic protein-2 (BMP-2) to support osteogenesis. Interestingly, we found that LAPONITE® promoted the FG-4592 induced upregulation of vascular endothelial growth factor (VEGF) gene expression of human umbilical cord endothelial cells (HUVECs). Additionally, we observed that LAPONITE® could provide a sustained release of BMP-2 and significantly potentiate the osteogenic effects of BMP-2 on adipose derived mesenchymal stem cells (AMSCs). Overall, current findings on the LAPONITE®-drug/protein model system provide a unique way to potentiate the angiogenic activities of FG-4592 on HUVECs and osteogenic effects of BMP-2 on AMSCs for tissue engineering application. Future studies will be directed towards gaining a deeper understanding of these effects on a co-culture system of HUVECs and AMSCs.

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LAPONITE® 纳米硅酸盐可增强 FG-4592 的血管生成效应和 BMP-2 的成骨效应。

由于 LAPONITE® 具有独特的离子和物理特性，因此基于 LAPONITE® 的给药系统具有许多优势。LAPONITE® 纳米粒子的高离子性和大表面积使其能够插层和溶解生物分子。在本研究中，我们探索了 LAPONITE® 作为 FG-4592 载体支持血管生成和作为骨形态发生蛋白-2 (BMP-2) 载体支持成骨的潜力。有趣的是，我们发现 LAPONITE® 促进了 FG-4592 诱导的人脐带内皮细胞（HUVECs）血管内皮生长因子（VEGF）基因表达的上调。此外，我们还观察到 LAPONITE® 可持续释放 BMP-2，并显著增强 BMP-2 对脂肪间充质干细胞 (AMSC) 的成骨效应。总之，目前关于 LAPONITE® 药物/蛋白质模型系统的研究结果为增强 FG-4592 对 HUVECs 的血管生成活性和 BMP-2 对 AMSCs 的成骨效应提供了一种独特的方法，可用于组织工程应用。未来的研究将致力于深入了解这些作用对 HUVECs 和 AMSCs 共培养系统的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomaterials Science MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

11.50

自引率

4.50%

发文量

556

期刊介绍： Biomaterials Science is an international high impact journal exploring the science of biomaterials and their translation towards clinical use. Its scope encompasses new concepts in biomaterials design, studies into the interaction of biomaterials with the body, and the use of materials to answer fundamental biological questions.