Electrochemical biosensing of cerium with a tyrosine-functionalized EF-hand loop peptide

IF 3.5 3区工程技术 Q2 ENGINEERING, CHEMICAL

AIChE Journal Pub Date : 2024-10-02 DOI:10.1002/aic.18620

Sogol Asaei, Geeta Verma, Nicholas S. Sinclair, Julie N. Renner

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Abstract

The significance of easily detecting rare earth elements (REEs) has increased due to the growing demand for REEs. Addressing this need, we present an innovative electrochemical biosensor, focusing on cerium as a model REE. This biosensor utilizes a modified EF-hand loop peptide sequence, incorporating cysteine for covalent attachment to a gold working electrode and tyrosine as an electrochemically active amino acid. The sensor was designed such that binding to cerium induces a conformational change in the peptide, affecting tyrosine's proximity to the electrode surface, modulating the current. A calibration curve was generated from cyclic voltammetry current peaks at ~0.55–0.65 V versus a silver pseudo-reference electrode, with cerium concentrations ranging from 0 to 67 μM in artificial urine. The sensor exhibited a biologically relevant limit of detection of 35 μM and a sensitivity of −0.0024 ± 0.002 (μA μM)⁻¹. These findings offer insights into designing peptide sequences for electrochemical biosensing.

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利用酪氨酸功能化的 EF 手环肽对铈进行电化学生物传感

由于对稀土元素（REEs）的需求日益增长，轻松检测稀土元素（REEs）的重要性也随之增加。为了满足这一需求，我们提出了一种创新的电化学生物传感器，并将铈作为稀土元素的模型。这种生物传感器采用了改进的 EF 手环肽序列，其中半胱氨酸用于与金工作电极共价连接，酪氨酸则作为电化学活性氨基酸。传感器的设计原理是，与铈结合后，肽的构象会发生变化，影响酪氨酸与电极表面的接近程度，从而调节电流。根据人工尿液中 0 至 67 μM 的铈浓度，在 ~0.55-0.65 V 处相对于银伪参比电极的循环伏安电流峰值生成了校准曲线。该传感器的生物相关检测限为 35 μM，灵敏度为 -0.0024 ± 0.002 (μA μM)-1。这些发现为设计用于电化学生物传感的肽序列提供了启示。

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来源期刊

AIChE Journal 工程技术-工程：化工

CiteScore

7.10

自引率

10.80%

发文量

411

审稿时长

3.6 months

期刊介绍： The AIChE Journal is the premier research monthly in chemical engineering and related fields. This peer-reviewed and broad-based journal reports on the most important and latest technological advances in core areas of chemical engineering as well as in other relevant engineering disciplines. To keep abreast with the progressive outlook of the profession, the Journal has been expanding the scope of its editorial contents to include such fast developing areas as biotechnology, electrochemical engineering, and environmental engineering. The AIChE Journal is indeed the global communications vehicle for the world-renowned researchers to exchange top-notch research findings with one another. Subscribing to the AIChE Journal is like having immediate access to nine topical journals in the field. Articles are categorized according to the following topical areas: Biomolecular Engineering, Bioengineering, Biochemicals, Biofuels, and Food Inorganic Materials: Synthesis and Processing Particle Technology and Fluidization Process Systems Engineering Reaction Engineering, Kinetics and Catalysis Separations: Materials, Devices and Processes Soft Materials: Synthesis, Processing and Products Thermodynamics and Molecular-Scale Phenomena Transport Phenomena and Fluid Mechanics.