Action of melatonin and physical exercise on the liver of cirrhotic rats: Study of oxidative stress and the inflammatory process.

IF 1.2 Q4 GASTROENTEROLOGY & HEPATOLOGY

Hepatology Forum Pub Date : 2024-09-01 eCollection Date: 2024-01-01 DOI:10.14744/hf.2023.2023.0037

Gabriela S Martins, Carlos Gustavo S Rosa, Elizangela G Schemitt, Josieli R Colares, Sandielly Rb Fonseca, Marilda S Brasil, Millena O Engeroff, Norma P Marroni

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Abstract

Background and aim: Cirrhosis is characterized by structural and functional alterations of the liver. Melatonin (MLT) has antioxidant properties. Physical exercise (EX) can reverse muscle loss in cirrhotic patients. The objective was to evaluate the action of MLT and EX on the liver of rats subjected to the experimental model of bile duct ligation (BLD).

Materials and methods: 48 male Wistar rats were used, divided into groups: Control (CO), CO+MLT, CO+EX, CO+MLT+EX, BDL, BDL+MLT, BDL+EX, and BDL+MLT+EX. The treatments occurred from the 15^th to the 28^th day. The dose of MLT was 20 mg/kg via I.p (1x/day), and the EX was performed 10 min/day. Blood and liver were collected for analysis.

Results: The liver integrity enzymes AST, ALT, and ALP showed a significant reduction in the groups treated with MLT and EX. Histological analyses showed reorganization of the liver parenchyma, reduction of inflammatory infiltrate, and fibrotic nodules. Lipoperoxidation (LPO), the activity of antioxidant enzymes, and nitric oxide metabolites showed a significant reduction in the groups treated with MLT and EX. The expression of TNF-α and NF-kB decreased in the treated groups.

Conclusion: Melatonin and physical exercise seem to be effective in restoring the parameters evaluated in this model of experimental cirrhosis.

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褪黑激素和体育锻炼对肝硬化大鼠肝脏的作用：氧化应激和炎症过程研究

背景和目的：肝硬化以肝脏结构和功能改变为特征。褪黑素（MLT）具有抗氧化作用。体育锻炼（EX）可逆转肝硬化患者的肌肉损失。材料和方法：使用 48 只雄性 Wistar 大鼠，分为对照组（CO）、CO+MLT 组（CO+MLT 组）和 EX 组（EX 组）：对照组（CO）、CO+MLT 组、CO+EX 组、CO+MLT+EX 组、BDL 组、BDL+MLT 组、BDL+EX 组和 BDL+MLT+EX 组。治疗时间为第 15 天至第 28 天。MLT的剂量为20毫克/千克，经静脉注射（1次/天），EX的剂量为10分钟/天。采集血液和肝脏进行分析：结果：接受 MLT 和 EX 治疗组的肝脏完整性酶 AST、ALT 和 ALP 显著降低。组织学分析表明，肝实质重组，炎症浸润和纤维化结节减少。脂肪过氧化（LPO）、抗氧化酶活性和一氧化氮代谢物在接受 MLT 和 EX 治疗的组别中显著减少。结论：结论：褪黑素和体育锻炼似乎能有效恢复实验性肝硬化模型中的评估指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hepatology Forum

CiteScore

1.90

自引率

12.50%

发文量