Elena Lovyagina, Oksana Luneva, Aleksey Loktyushkin, Boris Semin
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引用次数: 0
Abstract
In the region of slightly acidic pH (рН 5.7), the manganese cluster in oxygen-evolving complex of photosystem II (PSII) is more resistant to exogenous reductants. The effect of such pH on the heat inactivation efficiency of the electron transport chain (O2 evolution and 2,6-dichlorophenolindophenol reduction) in PSII membranes and thylakoid membranes was investigated. Under thylakoid membranes illumination accompanied by lumen acidification, their resistance to heat inactivation increases. In the presence of protonophores, the rate of heat inactivation increases, which seems to be associated not with the protonophore mechanism, but with structural and/or functional changes in membranes. In PSII membrane preparations, the efficiency of the oxygen evolution inhibition at pH 5.7 is also lower than at pH 6.5. The role of reactive oxygen species in thermal inactivation of photosynthetic membranes was investigated using a lipophilic cyclic hydroxylamine ESR spin probe.
期刊介绍：
The Journal of Plant Research is an international publication that gathers and disseminates fundamental knowledge in all areas of plant sciences. Coverage extends to every corner of the field, including such topics as evolutionary biology, phylogeography, phylogeny, taxonomy, genetics, ecology, morphology, physiology, developmental biology, cell biology, molecular biology, biochemistry, biophysics, bioinformatics, and systems biology.
The journal presents full-length research articles that describe original and fundamental findings of significance that contribute to understanding of plants, as well as shorter communications reporting significant new findings, technical notes on new methodology, and invited review articles.
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