Niraparib Enhances and Synergizes with Ganglioside GD2 to Potentiate its Inhibitory Effect on Bladder Cancer Cells.

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Combinatorial chemistry & high throughput screening Pub Date : 2024-10-01 DOI:10.2174/0113862073343602240909115320

Shan Lu, Xiaojia Gao, Xin Li, Hongchao Zhao, Hongda Lu

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Abstract

Purpose: This study aimed to study the inhibitory effect of niraparib alone or in combination with GD2 specific antibody on Bladder Cancer (BCa).

Methods: The migration ability of BCa cells was assessed through a scratch assay. CCK-8 assay was performed to evaluate the viability of BCa cells, and Transwell invasion assays were utilized to examine invasive capacity. The expression levels of E-cadherin and vimentin in BCa cells were measured using QRT-PCR.

Results: Western blot showed the EMT level to be the lowest in the niraparib+GD2 group. The transwell invasion assay suggested that the invasion ability of BCa cells was weakened in the niraparib+ GD2 group. CCK8 assay indicated that the proliferation ability of BCa cells was decreased. Scratch test suggested that the migration ability of BCa cells was weakened. PCR result showed that the niraparib + GD2 group had the most significant inhibitory effect on mRNA expression of EMT markers.

Conclusion: Niraparib combined with a GD2-specific antibody exerted a more prominent inhibitory effect on BCa.

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尼拉帕利能增强神经节苷脂 GD2 的抑制作用并与之协同增效。

目的：本研究旨在探讨尼拉帕利单独或与GD2特异性抗体联合使用对膀胱癌（BCa）的抑制作用：方法：通过划痕试验评估 BCa 细胞的迁移能力。方法：通过划痕试验评估 BCa 细胞的迁移能力，CCK-8 试验评估 BCa 细胞的活力，Transwell 侵袭试验检测细胞的侵袭能力。使用 QRT-PCR 检测了 BCa 细胞中 E-cadherin和波形蛋白的表达水平：Western印迹显示，尼拉帕利+GD2组的EMT水平最低。经孔侵袭试验表明，尼拉帕利+GD2组BCa细胞的侵袭能力减弱。CCK8检测表明，BCa细胞的增殖能力下降。划痕试验表明 BCa 细胞的迁移能力减弱。PCR结果显示，尼拉帕利+GD2组对EMT标志物mRNA表达的抑制作用最显著：结论：尼拉帕利联合GD2特异性抗体对BCa的抑制作用更为显著。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Combinatorial chemistry & high throughput screening 化学-生化研究方法

CiteScore

3.10

自引率

5.60%

发文量

327

审稿时长

7.5 months

期刊介绍： Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.