Ultrasound-assisted enzymatic extraction of jujube (Ziziphus jujuba Mill.) polysaccharides: Extraction efficiency, antioxidant activity, and structure features

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2024-09-28 DOI:10.1016/j.ultsonch.2024.107088

Yiting Guo , Shenao Nan , Chengcheng Qiu , Chenyu Song , Bengang Wu , Yanhua Tang , Lifang Cheng , Haile Ma

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Abstract

This study investigated the effect of ultrasound-assisted enzymatic extraction (UAEE) on the extraction efficiency, antioxidant activity, and structural properties of jujube polysaccharide (JPS), with hot water extraction (HWE), ultrasound-assisted extraction (UAE), and enzymatic-assisted extraction (EAE) serving as controls. Optimal extraction conditions were determined through a multi-index weighted scoring method that comprehensively accounted for yield, duration, and antioxidant activity. Results demonstrated that the JPS yield obtained by UAEE at 22/33 kHz was 10.5 % to 16.3 % higher than those achieved by the other methods, significantly enhancing antioxidant activity. Monosaccharide composition analysis revealed that UAEE increased the content of key mono-sugars in JPS. Additionally, assessments of molecular weight distribution, zeta potential, and rheological properties showed that UAEE reduced the molecular weight and apparent viscosity of JPS, resulting in a looser structural configuration. These structural modifications were observed in scanning electron microscope (SEM) images, which revealed a filamentous branched morphology in JPS obtained through UAEE. Further observations using the atomic force microscope (AFM) indicated that the polysaccharide chains extracted by UAEE were shorter in length, lower in height, and free from aggregation.

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超声辅助酶法提取大枣多糖：提取效率、抗氧化活性和结构特征

本研究以热水提取（HWE）、超声辅助提取（UAE）和酶解辅助提取（EAE）为对照，考察了超声辅助酶解提取（UAEE）对红枣多糖（JPS）的提取效率、抗氧化活性和结构特性的影响。通过多指标加权评分法确定了最佳提取条件，该方法综合考虑了产量、持续时间和抗氧化活性。结果表明，UAEE 在 22/33 kHz 下获得的 JPS 产量比其他方法高出 10.5% 至 16.3%，显著提高了抗氧化活性。单糖成分分析表明，UAEE 增加了 JPS 中关键单糖的含量。此外，对分子量分布、ZETA电位和流变特性的评估表明，UAEE降低了JPS的分子量和表观粘度，使其结构更松散。扫描电子显微镜（SEM）图像显示，通过 UAEE 获得的 JPS 呈丝状分枝形态。使用原子力显微镜（AFM）进行的进一步观察表明，通过 UAEE 提取的多糖链长度更短、高度更低，而且没有聚集现象。

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.