Antioxidant and anticancer activities on HT-29 colon cancer cells of protein isolate extracted from Cordyceps militaris fruiting body using diverse isolation methods

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry Pub Date : 2024-09-24 DOI:10.1016/j.procbio.2024.09.024

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引用次数: 0

Abstract

The present research examines different techniques for isolating proteins from Cordyceps militaris fruiting body powder (COR), namely ammonium sulfate precipitation (ASP), organic solvent precipitation (OSP), and isoelectric precipitation (IEP). ASP yielded the highest protein content (78.90 %), effectively concentrating proteins, while OSP and IEP also produced substantial yields (57.41 % and 69.06 %, respectively). Color analysis revealed differences among isolates, with ASP resulting in a darker hue. ASP and IEP showed higher red and yellow components, respectively. Essential amino acid content was significantly higher in ASP isolate compared to COR, indicating effective amino acid concentration. Thermal analysis revealed different decomposition temperatures and enthalpy values among isolates. OSP displayed the highest phenolic content, while IEP exhibited the highest flavonoid content. Antioxidant assays showed ASP to have the highest DPPH-RSA and ABTS-RSA, while IEP showed the highest FRAP value. ASP demonstrated anticancer activity against HT-29 colon cancer cells inducing cell death apoptosis and inhibiting cell migration, with an IC₅₀ of 360.63 µg/mL, comparable to Paclitaxel. These findings suggest that Cordyceps militaris protein isolate, particularly from ASP, has significant antioxidant and anticancer potential, supporting its potential as a natural alternative to synthetic drugs with fewer side effects.

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采用不同分离方法从冬虫夏草子实体中提取的分离蛋白对 HT-29 结肠癌细胞的抗氧化和抗癌活性

本研究探讨了从冬虫夏草子实体粉（COR）中分离蛋白质的不同技术，即硫酸铵沉淀法（ASP）、有机溶剂沉淀法（OSP）和等电沉淀法（IEP）。硫酸铵沉淀的蛋白质含量最高（78.90%），有效浓缩了蛋白质；有机溶剂沉淀和等电沉淀的蛋白质含量也很可观（分别为 57.41% 和 69.06%）。颜色分析显示了不同分离物之间的差异，ASP 的颜色较深。ASP 和 IEP 的红色和黄色成分分别较高。与 COR 相比，ASP 分离物的必需氨基酸含量明显较高，表明氨基酸浓度较高。热分析表明，不同分离物的分解温度和焓值不同。OSP 的酚含量最高，而 IEP 的类黄酮含量最高。抗氧化测定显示，ASP 的 DPPH-RSA 和 ABTS-RSA 值最高，而 IEP 的 FRAP 值最高。ASP 对 HT-29 结肠癌细胞具有抗癌活性，可诱导细胞凋亡并抑制细胞迁移，其 IC50 值为 360.63 µg/mL，与紫杉醇相当。这些研究结果表明，冬虫夏草蛋白分离物（尤其是 ASP）具有显著的抗氧化和抗癌潜力，支持其作为副作用较少的合成药物天然替代品的潜力。

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来源期刊

Process Biochemistry 生物-工程：化工

CiteScore

8.30

自引率

4.50%

发文量

374

审稿时长

53 days

期刊介绍： Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.