NQO1 from Litopenaeus vannamei involved in the regulation of antioxidant capacity, inflammation, and apoptosis

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-09-28 DOI:10.1016/j.aqrep.2024.102380

Shiping Yang, Yunhao Yuan, Cheng Chen, Junliang Luo, Guojian Li, Jichang Jian, Shuanghu Cai, Huiling Liu

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引用次数: 0

Abstract

NAD(P)H:quinone oxidoreductase 1 (NQO1) is a versatile FAD-dependent flavoprotein and a key regulator in the Nrf2/ARE signaling pathway. In this study, we cloned the NQO1 gene of Litopenaeus vannamei (LvNQO1) and investigated its role in immune regulation. The results showed that the LvNQO1 gene has an ORF of 987 bp, encoding 328 amino acids. The protein contains an NQO family domain. Homology and phylogenetic analysis revealed that LvNQO1 shares similarities with the NQO1 of Marsupenaeus japonicus, clustering into the same clade. LvNQO1 was expressed in 10 tissues of L. vannamei, with the highest expression in the hepatopancreas and the lowest in the intestine. After RNA interference (RNAi) of LvNQO1, the expression of antioxidant genes (SOD and GST) initially increased but then decreased after 24 h. The expression levels of Gpx and CAT decreased early on but gradually recovered. Immune-related factors (Toll4, JNK, and Hsp90) and apoptosis-related factors (Caspase3, P53, and bcl2) were upregulated. Following RNAi of LvNQO1, Vibrio harveyi challenge significantly increased the expression of immune factors (JNK and HIF1α) and apoptosis factors (Caspase3, P53, and bcl2) in the hepatopancreas. Toll 4 receptors recognized the infection, upregulated their expression, and activated an immune response. RNAi of LvNQO1 led to noticeable histological lesions and apoptosis. These findings suggest that LvNQO1 may play a role in regulating antioxidant capacity, inflammation, and apoptosis in response to Vibrio infection.

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万年青中的 NQO1 参与调节抗氧化能力、炎症和细胞凋亡

NAD(P)H:醌氧化还原酶1（NQO1）是一种依赖于FAD的多功能黄蛋白，也是Nrf2/ARE信号通路的关键调节因子。本研究克隆了凡纳滨对虾的 NQO1 基因（LvNQO1），并研究了其在免疫调节中的作用。结果表明，LvNQO1 基因的 ORF 为 987 bp，编码 328 个氨基酸。该蛋白含有一个 NQO 家族结构域。同源性和系统进化分析表明，LvNQO1与日本马苏藻的NQO1具有相似性，属于同一支系。LvNQO1 在凡纳滨鲤的 10 个组织中均有表达，其中肝胰腺的表达量最高，肠道的表达量最低。对 LvNQO1 进行 RNA 干扰（RNAi）后，抗氧化基因（SOD 和 GST）的表达量最初有所增加，但 24 小时后又有所下降。Gpx和CAT的表达水平在早期有所下降，但随后逐渐恢复。免疫相关因子（Toll4、JNK 和 Hsp90）和凋亡相关因子（Caspase3、P53 和 bcl2）上调。在对 LvNQO1 进行 RNAi 后，哈维弧菌挑战显著增加了肝胰腺中免疫因子（JNK 和 HIF1α）和凋亡因子（Caspase3、P53 和 bcl2）的表达。Toll 4受体识别感染，上调其表达，并激活免疫反应。LvNQO1 的 RNAi 导致了明显的组织学病变和细胞凋亡。这些研究结果表明，LvNQO1 可能在弧菌感染时起到调节抗氧化能力、炎症和细胞凋亡的作用。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.