Detection of Shiga toxin-producing Escherichia coli in dairy cows: genetic characterization and inhibition of adherence by cattle anti-STEC antibodies to HEp-2 cell.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Research Communications Pub Date : 2024-12-01 Epub Date: 2024-09-27 DOI:10.1007/s11259-024-10557-z

Rocío Colello, M Victoria Vélez, Marcelo Rodríguez, Ariel Rogé, Analía I Etcheverría, Nora Lía Padola

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引用次数: 0

Abstract

Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen associated with severe disease. Cattle are recognized as the primary animal reservoir of STEC. This study reports the occurrence and characterization of STEC from dairy cows and evaluates the inhibition of adherence by cattle anti-STEC antibodies to the HEp-2 cell. From 151 samples, 30% (n = 45) were positive for stx by PCR screening (25.21% of dairy cows and 46.8% of growing calves). From these positive samples, 17 STEC isolates were characterized. In dairy cows, 3 out of 17 samples carried stx2, 3 out of 17 possessed stx1, and 2 out of 17 carried stx1/stx2. In growing cows, 8 out of 17 samples carried stx1 and 1 out of 17 carried stx1/stx2. Other virulence factors such as ehxA, saa, iha, cah, and eae were detected. The strains were typed into 3 E. coli O groups (O26, O91, and O130). The analysis of the HEp-2 adherence assays indicated that all serum from both cattle categories presented high levels of inhibition of adherence. Considering the severity of the symptoms caused by STEC in humans, searching for factors influencing the colonization of STEC in cattle would help identify strategies to avoid transmission and STEC infection.

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检测奶牛中产志贺毒素的大肠杆菌：牛抗 STEC 抗体对 HEp-2 细胞粘附的基因特征和抑制作用。

产志贺毒素大肠杆菌（STEC）是一种与严重疾病相关的人畜共患病原体。牛被认为是 STEC 的主要动物贮藏库。本研究报告了奶牛 STEC 的发生和特征，并评估了牛抗 STEC 抗体对 HEp-2 细胞粘附的抑制作用。在 151 份样本中，30%（n = 45）的样本经 PCR 筛选后 STx 呈阳性（25.21% 的奶牛和 46.8% 的生长犊牛）。从这些阳性样本中，鉴定出 17 个 STEC 分离物。在奶牛样本中，17 个样本中有 3 个携带 stx2，3 个携带 stx1，2 个携带 stx1/stx2。在生长奶牛中，17 个样本中有 8 个携带 stx1，1 个携带 stx1/stx2。还检测到其他毒力因子，如 ehxA、saa、iha、cah 和 eae。这些菌株被分为 3 个大肠杆菌 O 组（O26、O91 和 O130）。HEp-2 吸附试验分析表明，来自两类牛的所有血清都具有较高的吸附抑制作用。考虑到 STEC 在人类中引起的症状的严重性，寻找影响 STEC 在牛中定植的因素将有助于确定避免传播和 STEC 感染的策略。

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来源期刊

Veterinary Research Communications 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

173

审稿时长

3 months

期刊介绍： Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.