The possible effects of chronic administration of amiodarone hydrochloride on the seminiferous tubules of adult male albino rats: histological and biochemical study.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-26 DOI:10.1080/01913123.2024.2407330

Amany M Abo-Ouf, Amany F Mohamed, Heba Abdelnaser Aboelsoud, Ayman Geddawy, Heba F Ibrahim

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Abstract

Amiodarone hydrochloride is an antiarrhythmic agent that is widely prescribed. However, it has serious side effects that approximately affect the whole body organs. In our study, we aimed to assess the possible effects of chronic administration of two different doses of amiodarone hydrochloride on the oxidative and inflammatory parameters as well as the histological morphology and ultrastructure of the seminiferous tubules of adult male albino rats. Forty rats were divided into four groups; Control group 1: each rat did not receive any drugs at all. Control group 2: each rat received 3 ml of 0.16% methylcellulose, orally and daily for 4 weeks. Low dose amiodarone group: each rat received 3 ml of 0.16% methylcellulose contained 3.6 mg amiodarone, orally and daily for 4 weeks. High dose amiodarone group: each rat received 3 ml of 0.16% methylcellulose contained 7.2 mg amiodarone, orally and daily for 4 weeks. Blood samples were collected for measuring serum levels of malondialdehyde, superoxide dismutase, interleukin-6 and tumor necrosis factor-alpha. Testes specimens were examined to assess the morphological changes and the level of expression of caspase-3 apoptotic marker. The results indicated that; amiodarone hydrochloride could induce a dose-dependent toxicity, causing oxidative stress, inflammation, cellular degeneration, deposition of collagen and enhanced apoptosis in the seminiferous tubules.

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长期服用盐酸胺碘酮对成年雄性白化大鼠曲细精管的可能影响：组织学和生化研究。

盐酸胺碘酮是一种抗心律失常药物，被广泛使用。然而，它具有严重的副作用，可影响全身器官。在我们的研究中，我们旨在评估长期服用两种不同剂量的盐酸胺碘酮对成年雄性白化大鼠曲细精管的氧化和炎症参数以及组织学形态和超微结构可能产生的影响。将 40 只大鼠分为四组：对照组 1：每只大鼠不接受任何药物。对照组 2：每只大鼠每天口服 3 毫升 0.16% 甲基纤维素，连续 4 周。低剂量胺碘酮组：每只大鼠每天口服 3 毫升含 3.6 毫克胺碘酮的 0.16%甲基纤维素，连续 4 周。高剂量胺碘酮组：每只大鼠每天口服 3 毫升含 7.2 毫克胺碘酮的 0.16%甲基纤维素，连续 4 周。收集血液样本以测量血清中丙二醛、超氧化物歧化酶、白细胞介素-6 和肿瘤坏死因子-α 的水平。对睾丸标本进行检查，以评估形态学变化和 caspase-3 细胞凋亡标记物的表达水平。结果表明，盐酸胺碘酮可诱发剂量依赖性毒性，导致氧化应激、炎症、细胞变性、胶原沉积和曲细精管细胞凋亡增强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.