Multidrug-Resistant Proteus mirabilis and Other Gram-Negative Species Isolated from Native Egyptian Chicken Carcasses.

IF 2.8 4区 医学 Q2 INFECTIOUS DISEASES
Bassant Ashraf El-Saeed, Hend Ali Elshebrawy, Amira Ibrahim Zakaria, Adel Abdelkhalek, Kálmán Imre, Adriana Morar, Viorel Herman, Khalid Ibrahim Sallam
{"title":"Multidrug-Resistant <i>Proteus mirabilis</i> and Other Gram-Negative Species Isolated from Native Egyptian Chicken Carcasses.","authors":"Bassant Ashraf El-Saeed, Hend Ali Elshebrawy, Amira Ibrahim Zakaria, Adel Abdelkhalek, Kálmán Imre, Adriana Morar, Viorel Herman, Khalid Ibrahim Sallam","doi":"10.3390/tropicalmed9090217","DOIUrl":null,"url":null,"abstract":"<p><p>Poultry carcasses may be reservoirs for the zoonotic transmission of antimicrobial-resistant bacteria to humans and pose a major public health hazard. During the isolation of <i>Salmonella</i> from poultry and other foods, many of the presumptive typical <i>Salmonella</i> colonies on xylose lysine deoxycholate (XLD) agar were found to lack the <i>invA</i> gene, which is the specific target gene for <i>Salmonella</i> spp. Therefore, the current study aimed to estimate the prevalence and antimicrobial resistance profiles of extensively drug-resistant <i>invA</i>-negative non-<i>Salmonella</i> isolates recovered from native Egyptian chicken carcasses as presumptive <i>Salmonella</i> colonies on XLD agar. The non-<i>Salmonella</i> isolates were detected in 84% (126/150) of the examined native Egyptian chicken carcasses and classified into five genera, with prevalence rates of 64% (96/150), 14% (21/150), 6.7% (10/150), 3.3% (5/150), and 1.3% (2/150) for <i>Proteus</i>, <i>Citrobacter</i>, <i>Shigella</i>, <i>Pseudomonas</i>, and <i>Edwardsiella</i>, respectively. One hundred and ninety-five <i>invA</i>-negative, non-verified presumptive <i>Salmonella</i> isolates were recovered and classified at the species level into <i>Proteus mirabilis</i> (132/195; 67.7%), <i>Proteus vulgaris</i> (11/195; 5.6%), <i>Citrobacter freundii</i> (26/195; 13.3%), <i>Shigella flexneri</i> (8/195; 4.1%), <i>Shigella sonnei</i> (6/195; 3.1%), <i>Shigella dysenteriae</i> (3/195; 1.5%), <i>Pseudomonas fluorescens</i> (6/195; 3.1%), and <i>Edwardsiella tarda</i> (3/195; 1.5%). All (195/195; 100%) of these isolates showed resistance against cefaclor and fosfomycin. Additionally, these isolates showed high resistance rates of 98%, 92.8%, 89.7%, 89.2%, 89.2%, 86.7%, 80%, 78.5%, 74.4%, and 73.9% against cephalothin, azithromycin, vancomycin, nalidixic acid, tetracycline, sulfamethoxazole/trimethoprim, cefepime, gentamicin, cefotaxime, and ciprofloxacin, respectively. Interestingly, all (195/195; 100%) of the identified isolates were resistant to at least five antibiotics and exhibited an average MAR (multiple antibiotic resistance) index of 0.783. Furthermore, 73.9% of the examined isolates were classified as extensively drug-resistant, with an MAR index equal to 0.830. The high prevalence of extensively drug-resistant foodborne <i>Proteus</i>, <i>Citrobacter</i>, <i>Shigella</i>, <i>Pseudomonas</i>, and <i>Edwardsiella</i> isolated from native chicken carcasses poses a great hazard to public health and necessitates more monitoring and concern about the overuse and misuse of antibiotics in humans and animals. This study also recommends the strict implementation of GHP (good hygienic practices) and GMP (good manufacturing practices) in the chicken meat supply chain to protect consumer health.</p>","PeriodicalId":23330,"journal":{"name":"Tropical Medicine and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11436119/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Medicine and Infectious Disease","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/tropicalmed9090217","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

Abstract

Poultry carcasses may be reservoirs for the zoonotic transmission of antimicrobial-resistant bacteria to humans and pose a major public health hazard. During the isolation of Salmonella from poultry and other foods, many of the presumptive typical Salmonella colonies on xylose lysine deoxycholate (XLD) agar were found to lack the invA gene, which is the specific target gene for Salmonella spp. Therefore, the current study aimed to estimate the prevalence and antimicrobial resistance profiles of extensively drug-resistant invA-negative non-Salmonella isolates recovered from native Egyptian chicken carcasses as presumptive Salmonella colonies on XLD agar. The non-Salmonella isolates were detected in 84% (126/150) of the examined native Egyptian chicken carcasses and classified into five genera, with prevalence rates of 64% (96/150), 14% (21/150), 6.7% (10/150), 3.3% (5/150), and 1.3% (2/150) for Proteus, Citrobacter, Shigella, Pseudomonas, and Edwardsiella, respectively. One hundred and ninety-five invA-negative, non-verified presumptive Salmonella isolates were recovered and classified at the species level into Proteus mirabilis (132/195; 67.7%), Proteus vulgaris (11/195; 5.6%), Citrobacter freundii (26/195; 13.3%), Shigella flexneri (8/195; 4.1%), Shigella sonnei (6/195; 3.1%), Shigella dysenteriae (3/195; 1.5%), Pseudomonas fluorescens (6/195; 3.1%), and Edwardsiella tarda (3/195; 1.5%). All (195/195; 100%) of these isolates showed resistance against cefaclor and fosfomycin. Additionally, these isolates showed high resistance rates of 98%, 92.8%, 89.7%, 89.2%, 89.2%, 86.7%, 80%, 78.5%, 74.4%, and 73.9% against cephalothin, azithromycin, vancomycin, nalidixic acid, tetracycline, sulfamethoxazole/trimethoprim, cefepime, gentamicin, cefotaxime, and ciprofloxacin, respectively. Interestingly, all (195/195; 100%) of the identified isolates were resistant to at least five antibiotics and exhibited an average MAR (multiple antibiotic resistance) index of 0.783. Furthermore, 73.9% of the examined isolates were classified as extensively drug-resistant, with an MAR index equal to 0.830. The high prevalence of extensively drug-resistant foodborne Proteus, Citrobacter, Shigella, Pseudomonas, and Edwardsiella isolated from native chicken carcasses poses a great hazard to public health and necessitates more monitoring and concern about the overuse and misuse of antibiotics in humans and animals. This study also recommends the strict implementation of GHP (good hygienic practices) and GMP (good manufacturing practices) in the chicken meat supply chain to protect consumer health.

从埃及本地鸡肉中分离出的耐多药变形杆菌和其他革兰氏阴性菌种
家禽尸体可能是人畜共患耐抗菌素细菌传播给人类的贮藏库,对公共健康构成重大危害。从家禽和其他食物中分离沙门氏菌时,发现许多在木糖赖氨酸脱氧胆酸盐(XLD)琼脂上的推定典型沙门氏菌菌落缺乏 invA 基因,而 invA 基因是沙门氏菌属的特异性靶基因。因此,本研究旨在估算从埃及本地鸡尸体中分离出的广泛耐药的 invA 阴性非沙门氏菌分离物的流行率和抗菌谱,因为这些分离物是 XLD 琼脂上的推定沙门氏菌菌落。在84%(126/150)受检的埃及本地鸡尸体中检测到了非沙门氏菌分离物,并将其分为五个菌属,变形杆菌属、柠檬酸杆菌属、志贺氏菌属、假单胞菌属和爱德华氏菌属的流行率分别为64%(96/150)、14%(21/150)、6.7%(10/150)、3.3%(5/150)和1.3%(2/150)。回收的 195 例 invA 阴性、未经验证的推定沙门氏菌分离物在菌种水平上分为奇异变形杆菌(132/195;67.7%)、普通变形杆菌(11/195;5.6%)、弗氏柠檬酸杆菌(26/195;13.3%)、柔性志贺氏菌(8/195;4.1%)、宋内志贺氏菌(6/195;3.1%)、痢疾志贺氏菌(3/195;1.5%)、荧光假单胞菌(6/195;3.1%)和塔尔达爱德华氏菌(3/195;1.5%)。所有这些分离物(195/195;100%)都对头孢克洛和磷霉素产生了耐药性。此外,这些分离物对头孢菌素、阿奇霉素、万古霉素、萘啶酸、四环素、磺胺甲恶唑/三甲氧苄啶、头孢吡肟、庆大霉素、头孢噻肟、环丙沙星的耐药率分别为 98%、92.8%、89.7%、89.2%、89.2%、86.7%、80%、78.5%、74.4% 和 73.9%。有趣的是,所有(195/195;100%)被鉴定的分离物都对至少五种抗生素具有耐药性,平均耐多种抗生素指数(MAR)为 0.783。此外,73.9%的受检分离菌株被归类为广泛耐药菌株,MAR指数为0.830。从本地鸡胴体中分离出的食源性变形杆菌、柠檬酸杆菌、志贺氏菌、假单胞菌和爱德华氏菌广泛耐药的高流行率对公众健康造成了极大的危害,因此有必要对人类和动物抗生素的过度使用和滥用进行更多的监测和关注。这项研究还建议在鸡肉供应链中严格执行 GHP(良好卫生规范)和 GMP(良好生产规范),以保护消费者的健康。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Tropical Medicine and Infectious Disease
Tropical Medicine and Infectious Disease Medicine-Public Health, Environmental and Occupational Health
CiteScore
3.90
自引率
10.30%
发文量
353
审稿时长
11 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信