Fine mapping and identification of ERF transcription factor ERF017 as a candidate gene for cold tolerance in pumpkin.

IF 4.4 1区 农林科学 Q1 AGRONOMY
Yarong Liao, Xiaoying Liu, Na Xu, Guangling Chen, Xinhui Qiao, Qinsheng Gu, Yu Wang, Jin Sun
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引用次数: 0

Abstract

Key message: Two major QTLs for cold tolerance in pumpkin were localised, and CmoERF017 was identified as a key candidate gene within these QTLs via RNA-seq. Functional analysis revealed that CmoERF017 was a positive regulator of pumpkin in response to low-temperature stress. Low temperature is a key environmental factor that affects the protected cultivation of cucumber (Cucumis sativus L.) in winter, and the cold tolerance of cucumber/pumpkin-grafted seedlings depends on the rootstock. Pumpkin (Cucurbita spp.) has a well-developed root system, high resistance and wide adaptation, commonly used as rootstock for cucumber to improve the cold tolerance of grafted seedlings. This study used two high-generation inbred lines of Cucurbita moschata with significant differences in cold tolerance. We identified key candidate genes within the major cold tolerance QTL of rootstocks using QTL-seq and RNA-seq and investigated the function and molecular mechanisms of these genes in response to low-temperature stress. Results showed that QTL-seq located two cold tolerance QTLs, qCII-1 and qCII-2, while RNA-seq located 28 differentially expressed genes within these QTLs. CmoERF017 was finally identified as a key candidate gene. Functional validation results indicated that CmoERF017 is a positive regulator of pumpkin in response to low-temperature stress and affected root ABA synthesis and signalling by directly regulating the expression of SDR7 and ABI5. This study identified a key gene for low-temperature stress tolerance in rootstock pumpkin and clarified its role in the molecular mechanism of hormone-mediated plant cold tolerance. The study findings enrich the theoretical understanding of low-temperature stress tolerance in pumpkin and are valuable for the selection and breeding of cold-tolerant varieties of pumpkin used for rootstocks.

ERF转录因子ERF017作为南瓜耐寒候选基因的精细图谱绘制和鉴定。
关键信息:通过RNA-seq定位了南瓜耐寒性的两个主要QTLs,并确定CmoERF017是这些QTLs中的一个关键候选基因。功能分析显示,CmoERF017是南瓜应对低温胁迫的正调控因子。低温是影响黄瓜(Cucumis sativus L.)冬季保护地栽培的关键环境因素,而黄瓜/南瓜嫁接苗的耐寒性取决于砧木。南瓜(Cucurbita spp.)根系发达,抗性强,适应性广,常用作黄瓜的砧木,以提高嫁接苗的耐寒性。本研究使用了两个耐寒性差异显著的葫芦高代近交系。我们利用QTL-seq和RNA-seq鉴定了砧木主要耐寒QTL中的关键候选基因,并研究了这些基因在响应低温胁迫时的功能和分子机制。结果表明,QTL-seq定位了两个耐寒QTL,即qCII-1和qCII-2,而RNA-seq定位了这些QTL中的28个差异表达基因。最终确定CmoERF017为关键候选基因。功能验证结果表明,CmoERF017是南瓜应对低温胁迫的正调控因子,通过直接调控SDR7和ABI5的表达影响根系ABA的合成和信号传导。该研究发现了砧木南瓜耐低温胁迫的关键基因,阐明了其在激素介导的植物耐低温分子机制中的作用。研究结果丰富了对南瓜耐低温胁迫的理论认识,对选育耐低温砧木南瓜品种具有重要价值。
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来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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