Reproductive development in Trithuria submersa (Hydatellaceae: Nymphaeales): the involvement of AGAMOUS-like genes.

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-09-26 DOI:10.1007/s00425-024-04537-5
Silvia Moschin, Sebastiano Nigris, Elisabetta Offer, Nicola Babolin, Adriana Chiappetta, Leonardo Bruno, Barbara Baldan
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引用次数: 0

Abstract

Main conclusion: In the early diverging angiosperm Trithuria submersa TsAG1 and TsAG2 are expressed in different flower organs, including bracts, while TsAG3 is more ovule-specific, probably functioning as a D-type gene. Species of Trithuria, the only genus of the family Hydatellaceae, represent ideal candidates to explore the biology and flower evolution of early diverging angiosperms. The life cycle of T. submersa is generally known, and the "reproductive units" are morphologically well described, but the availability of genetic and developmental data of T. submersa is still scarce. To fill this gap, a transcriptome analysis of the reproductive structures was performed and presented in this work. This analysis provided sequences of MADS-box transcription factors, a gene family known to be involved in flower and fruit development. In situ hybridization experiments on floral buds were performed to describe the spatiotemporal expression patterns of the AGAMOUS genes, revealing the existence of three AG genes with different expression domains in flower organs and in developing ovules. Trithuria may offer important clues to the evolution of reproductive function among early angiosperms and Nymphaeales in particular, and this study aims to broaden relevant knowledge regarding key genes of reproductive development in non-model angiosperms, shaping first flower appearance and evolution.

潜龙藻(Hydatellaceae: Nymphaeales)的生殖发育:类 AGAMOUS 基因的参与。
主要结论在早期分化的被子植物Trithuria submersa中,TsAG1和TsAG2在不同的花器官(包括苞片)中表达,而TsAG3更具有胚珠特异性,可能作为D型基因发挥作用。Trithuria是水螅科唯一的属,其物种是探索被子植物早期分化的生物学和花进化的理想候选物种。潜龙草的生命周期已广为人知,其 "生殖单位 "在形态学上也有很好的描述,但潜龙草的遗传和发育数据仍然很少。为了填补这一空白,本研究对生殖结构进行了转录组分析。该分析提供了 MADS-box 转录因子的序列,MADS-box 是一个已知参与花和果实发育的基因家族。为了描述 AGAMOUS 基因的时空表达模式,对花蕾进行了原位杂交实验,结果发现在花器官和发育中的胚珠中存在三个具有不同表达域的 AG 基因。三叶草可能为早期被子植物,特别是仙人掌科植物的生殖功能进化提供了重要线索,该研究旨在拓宽非模式被子植物生殖发育关键基因的相关知识,从而影响最初花的出现和进化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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