Regulatory mechanisms of miR171d-SCL6 module in the rooting process of Acer rubrum L.

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-09-28 DOI:10.1007/s00425-024-04539-3
Huiju Li, Jiayu Yu, Jiaming Qin, Hewen Zhao, Kezhong Zhang, Wei Ge
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引用次数: 0

Abstract

Main conclusion: MiR171d and SCL6 are induced by the plant hormone auxin. MiR171d negatively regulates the expression of SCL6, thereby regulating the growth and development of plant adventitious roots. Under natural conditions, it is difficult to induce rooting in the process of propagating Acer rubrum L. via branches, which seriously limits its wide application in landscaping construction. In this study, the expression of Ar-miR171d was downregulated and the expression of ArSCL6 was upregulated after 300 mg/L indole-3-butyric acid (IBA) treatment. The transient interaction of Ar-miR171d and ArSCL6 in tobacco cells further confirmed their cleavage activity. Transgenic function verification confirmed that OE-Ar-miR171d inhibited adventitious root (AR) development, while OE-ArSCL6 promoted AR development. Tissue-specific expression verification of the ArSCL6 promoter demonstrated that it was specifically expressed in the plant root and leaf organs. Subcellular localization and transcriptional activation assays revealed that both ArSCL6 and ArbHLH089 were located in the nucleus and exhibited transcriptional activation activity. The interaction between the two was verified by bimolecular fluorescence complementarity (BIFC) experiments. These results help elucidate the regulatory mechanisms of the Ar-miR171d-ArSCL6 module during the propagation of A. rubrum and provide a molecular basis for the rooting of branches.

miR171d-SCL6 模块在红叶复叶槭生根过程中的调控机制
主要结论MiR171d和SCL6受植物激素辅助素的诱导。MiR171d 负向调节 SCL6 的表达,从而调节植物不定根的生长和发育。在自然条件下,红叶复叶槭(Acer rubrum L.)通过枝条繁殖的过程中很难诱导生根,这严重限制了其在园林绿化建设中的广泛应用。本研究发现,吲哚-3-丁酸(IBA)处理 300 mg/L 后,Ar-miR171d 的表达下调,ArSCL6 的表达上调。Ar-miR171d 和 ArSCL6 在烟草细胞中的瞬时相互作用进一步证实了它们的裂解活性。转基因功能验证证实,OE-Ar-miR171d抑制不定根(AR)的发育,而OE-ArSCL6则促进AR的发育。ArSCL6 启动子的组织特异性表达验证表明,它在植物根部和叶片器官中特异性表达。亚细胞定位和转录激活实验表明,ArSCL6 和 ArbHLH089 都位于细胞核中,并表现出转录激活活性。二者之间的相互作用通过双分子荧光互补(BIFC)实验得到了验证。这些结果有助于阐明 Ar-miR171d-ArSCL6 模块在红叶石蒜繁殖过程中的调控机制,并为枝条生根提供了分子基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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