Functional diversification of Sec13 isoforms for storage protein trafficking in rice endosperm cells.

IF 6.5 1区 生物学 Q1 PLANT SCIENCES
Yongfei Wang, Yulong Ren, Xuan Teng, Fan Wang, Yanyu Chen, Erchao Duan, Xin Wang, Tian Pan, Binglei Zhang, Gexing Wan, Yu Zhang, Pengcheng Zhang, Xiejun Sun, Wenkun Yang, Yun Zhu, Yu Chen, Wenjie Zhao, Xiaohang Han, Cailin Lei, Shanshan Zhu, Shijia Liu, Yihua Wang, Jianmin Wan
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Abstract

Coat protein complex II (COPII) vesicles play crucial roles in mediating the endoplasmic reticulum (ER) exit of newly synthesized proteins to the Golgi in eukaryotic cells. However, the molecular functions of COPII components and their functional diversifications in plant seeds remain obscure. Here, we showed that the rice (Oryza sativa) glutelin precursor accumulation12 (gpa12) mutant is defective in storage protein export from the ER, resulting in the formation of aggregated protein bodies. Map-based cloning revealed that GPA12 encodes a COPII outer layer protein, Sec13a, that mainly localizes to endoplasmic reticulum exit sites (ERES) and partially localizes to the Golgi. Biochemical experiments verified that Sec13a physically interacts with Sec31 and Sec16, and mutation in Sec13 compromises its interaction with Sec31 and Sec16, thereby affecting the membrane association of the inner complex components Sar1b and Sec23c. Apart from Sec13a, the rice genome encodes 2 other Sec13 isoforms, Sec13b and Sec13c. Notably, we observed an abnormal accumulation of globular ER structures in the sec13bc double mutant but not in the single mutants, suggesting a functional redundancy of Sec13b and Sec13c in modulating ER morphology. Taken together, our results substantiated that Sec13a plays an important role in regulating storage protein export from the ER, while Sec13b and Sec13c are required for maintaining ER morphology in rice endosperm cells. Our findings provide insights into the functional diversification of COPII components in plants.

Sec13 异构体在水稻胚乳细胞中运输贮藏蛋白的功能多样化
在真核细胞中,衣壳蛋白复合体 II(COPII)囊泡在介导新合成的蛋白质从内质网(ER)进入高尔基体的过程中起着至关重要的作用。然而,COPII 成分的分子功能及其在植物种子中的功能多样性仍不清楚。在这里,我们发现水稻(Oryza sativa)谷蛋白前体积累12(gpa12)突变体在从ER输出贮存蛋白方面存在缺陷,导致形成聚集的蛋白体。基于图谱的克隆发现,GPA12 编码一种 COPII 外层蛋白 Sec13a,它主要定位于内质网出口位点(ERES),部分定位于高尔基体。生化实验证实,Sec13a与Sec31和Sec16有物理相互作用,Sec13的突变会影响其与Sec31和Sec16的相互作用,从而影响内复合物成分Sar1b和Sec23c的膜结合。除了 Sec13a 之外,水稻基因组还编码了另外两种 Sec13 异构体:Sec13b 和 Sec13c。值得注意的是,我们在sec13bc双突变体中观察到球状ER结构的异常积累,而在单突变体中却没有观察到,这表明Sec13b和Sec13c在调节ER形态方面存在功能冗余。综上所述,我们的研究结果证实,Sec13a在调节ER中储存蛋白的输出方面起着重要作用,而Sec13b和Sec13c则是维持水稻胚乳细胞中ER形态的必要条件。我们的研究结果为了解植物中 COPII 成分的功能多样性提供了见解。
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来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
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