Effects of cell culture time and cytokines on migration of dental pulp stem cell-derived chondrogenic cells in collagen hydrogels.

IF 2.2 Q3 PHYSIOLOGY

Physiological Reports Pub Date : 2024-09-01 DOI:10.14814/phy2.70063

Li Yao, Nikol Flynn, Pranita Kaphle

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引用次数: 0

Abstract

The transplantation of collagen hydrogels encapsulating human dental pulp stem cell (DPSC)-derived chondrogenic cells is potentially a novel approach for the regeneration of degenerated nucleus pulposus (NP) and cartilage. Grafted cell migration allows cells to disperse in the hydrogels and the treated tissue from the grafted location. We previously reported the cell migration in type I and type II hydrogels. It is important to explore further how cell culture time affect the cell motility. In this study, we observed the decreased motility of DPSC-derived chondrogenic cells after culturing for 2 weeks compared with cells cultured for 2 days in these gels. The Alamarblue assay showed the cell proliferation during the two-week cell culture period. The findings suggest that the transitions of cell motility and proliferation during the longer culture time. The result indicates that the early culture stage is an optimal time for cell transplantation. In a degenerated disc, the expression of IL-1β and TNFα increased significantly compared with healthy tissue and therefore may affect grafted cell migration. The incorporation of IL-1β and TNFα into the collagen hydrogels decreased cell motility. The study indicates that the control of IL-1β and TNFα production may help to maintain cell motility after transplantation.

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细胞培养时间和细胞因子对牙髓干细胞衍生的软骨细胞在胶原水凝胶中迁移的影响

移植包裹人牙髓干细胞（DPSC）衍生的软骨细胞的胶原水凝胶是一种潜在的再生退化髓核（NP）和软骨的新方法。移植细胞迁移可使细胞从移植位置分散到水凝胶和治疗组织中。我们以前曾报道过细胞在 I 型和 II 型水凝胶中的迁移。进一步探讨细胞培养时间对细胞迁移的影响非常重要。在本研究中，我们观察到与在这些凝胶中培养 2 天的细胞相比，培养 2 周的 DPSC 衍生软骨细胞的运动能力下降了。阿拉玛蓝检测显示，细胞在两周的细胞培养期间出现了增殖。研究结果表明，细胞的运动和增殖在较长的培养时间内发生了转变。结果表明，早期培养阶段是细胞移植的最佳时机。在退化的椎间盘中，IL-1β和TNFα的表达量比健康组织显著增加，因此可能会影响移植细胞的迁移。在胶原水凝胶中加入IL-1β和TNFα会降低细胞的运动能力。研究表明，控制IL-1β和TNFα的产生可能有助于保持移植后细胞的运动性。

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来源期刊

Physiological Reports PHYSIOLOGY-

CiteScore

4.20

自引率

4.00%

发文量

374

审稿时长

9 weeks

期刊介绍： Physiological Reports is an online only, open access journal that will publish peer reviewed research across all areas of basic, translational, and clinical physiology and allied disciplines. Physiological Reports is a collaboration between The Physiological Society and the American Physiological Society, and is therefore in a unique position to serve the international physiology community through quick time to publication while upholding a quality standard of sound research that constitutes a useful contribution to the field.