{"title":"A novel, non-toxic chitosan modified with lysine to enhance buffer capacity for siRNA delivery.","authors":"Tianhui Liu, Mei Lin, Chengfei Zhao, Aizhu Lin","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The present study employed lysine as a modifying agent for chitosan (CS) to synthesise a novel CS derivative (LGCS) intended for siRNA delivery. The successful grafting of lysine to CS was characterized using FT-IR and the introduction of the lysine moiety resulted in improved solubility and buffering capacity of CS. The Zeta potential and size of LGCS/siRNA nanoparticles (NPs) were evaluated using dynamic light scattering (DLS) and the results were verified by transmission electron microscopy (TEM). Evaluation of LGCS's siRNA binding capacity was conducted using a gel retardation assay. The results showed that LGCS could effectively bind to siRNA and form a complex with a hydrated diameter of about 97.2 ± 1.3 nm. Furthermore, cytotoxicity assays conducted on RSC96 cells demonstrated that LGCS exhibited lower toxicity compared to linear polyethyleneimine (PEI) 25k. In vitro, cellular uptake assays also revealed that LGCS displayed excellent transfection efficiency. The results of our study lead us to the conclusion that LGCS holds great promise as a gene delivery vector.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pakistan journal of pharmaceutical sciences","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0
Abstract
The present study employed lysine as a modifying agent for chitosan (CS) to synthesise a novel CS derivative (LGCS) intended for siRNA delivery. The successful grafting of lysine to CS was characterized using FT-IR and the introduction of the lysine moiety resulted in improved solubility and buffering capacity of CS. The Zeta potential and size of LGCS/siRNA nanoparticles (NPs) were evaluated using dynamic light scattering (DLS) and the results were verified by transmission electron microscopy (TEM). Evaluation of LGCS's siRNA binding capacity was conducted using a gel retardation assay. The results showed that LGCS could effectively bind to siRNA and form a complex with a hydrated diameter of about 97.2 ± 1.3 nm. Furthermore, cytotoxicity assays conducted on RSC96 cells demonstrated that LGCS exhibited lower toxicity compared to linear polyethyleneimine (PEI) 25k. In vitro, cellular uptake assays also revealed that LGCS displayed excellent transfection efficiency. The results of our study lead us to the conclusion that LGCS holds great promise as a gene delivery vector.
期刊介绍：
Pakistan Journal of Pharmaceutical Sciences (PJPS) is a peer reviewed multi-disciplinary pharmaceutical sciences journal. The PJPS had its origin in 1988 from the Faculty of Pharmacy, University of Karachi as a biannual journal, frequency converted as quarterly in 2005, and now PJPS is being published as bi-monthly from January 2013.
PJPS covers Biological, Pharmaceutical and Medicinal Research (Drug Delivery, Pharmacy Management, Molecular Biology, Biochemical, Pharmacology, Pharmacokinetics, Phytochemical, Bio-analytical, Therapeutics, Biotechnology and research on nano particles.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
