Antigenic reactivity of Leishmania (Viannia) lainsoni axenic amastigote proved to be a suitable alternative for optimizing Montenegro skin test.

IF 3 2区医学 Q1 PARASITOLOGY

Parasites & Vectors Pub Date : 2024-09-27 DOI:10.1186/s13071-024-06486-0

Leonardo Viana de Melo, Thiago Vasconcelos Dos Santos, Patrícia Karla Ramos, Luciana Vieira Lima, Marliane Batista Campos, Fernando Tobias Silveira

{"title":"Antigenic reactivity of Leishmania (Viannia) lainsoni axenic amastigote proved to be a suitable alternative for optimizing Montenegro skin test.","authors":"Leonardo Viana de Melo, Thiago Vasconcelos Dos Santos, Patrícia Karla Ramos, Luciana Vieira Lima, Marliane Batista Campos, Fernando Tobias Silveira","doi":"10.1186/s13071-024-06486-0","DOIUrl":null,"url":null,"abstract":"Background: Laboratory diagnosis of American cutaneous leishmaniasis (ACL) requires a tool amenable to the epidemiological status of ACL in Brazil. Montenegro skin test (MST), an efficient immunological tool used for laboratory diagnosis of ACL, induces delayed-type hypersensitivity (DTH) response to the promastigote antigens of Leishmania; however, human immune responses against infection are modulated by the amastigote of the parasite. Leishmania (V.) lainsoni induces strong cellular immunity in humans; therefore, the antigenic reactivity of its axenic amastigote (AMA antigen) to MST was evaluated for the laboratory diagnosis of ACL.Methods: Among 70 individuals examined, 60 had a laboratory-confirmed diagnosis of ACL; 53 had localized cutaneous leishmaniasis (LCL), and 7 had mucosal leishmaniasis (ML). Patients were treated at the Evandro Chagas Institute's leishmaniasis clinic, Pará State, Brazil. Ten healthy individuals with no history of ACL (control group) were also examined. Leishmania (V.) braziliensis promastigote antigen (PRO) was used to compare the reactivity with that of AMA antigen. Paired Student's t-test, kappa agreement, and Spearman test were used to evaluate the reactivity of AMA and PRO.Results: The mean reactivity of AMA in ACL patients was 19.4 mm ± 13.3, which was higher (P < 0.001) than that of PRO: 12.1 mm ± 8.1. MST reactivity according to the clinical forms revealed that AMA reactivity in LCL and ML, 18.8 mm ± 13.3 and 24.3 mm ± 13.7, was higher (P < 0.001) than that of PRO, 11.8 mm ± 8.2 and 14.6 mm ± 8.4, respectively.Conclusion: AMA reactivity was higher than that of PRO, indicating that AMA is a promising alternative for optimizing MST in the laboratory diagnosis of ACL.","PeriodicalId":19793,"journal":{"name":"Parasites & Vectors","volume":"17 1","pages":"402"},"PeriodicalIF":3.0000,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11438107/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Parasites & Vectors","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s13071-024-06486-0","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Laboratory diagnosis of American cutaneous leishmaniasis (ACL) requires a tool amenable to the epidemiological status of ACL in Brazil. Montenegro skin test (MST), an efficient immunological tool used for laboratory diagnosis of ACL, induces delayed-type hypersensitivity (DTH) response to the promastigote antigens of Leishmania; however, human immune responses against infection are modulated by the amastigote of the parasite. Leishmania (V.) lainsoni induces strong cellular immunity in humans; therefore, the antigenic reactivity of its axenic amastigote (AMA antigen) to MST was evaluated for the laboratory diagnosis of ACL.

Methods: Among 70 individuals examined, 60 had a laboratory-confirmed diagnosis of ACL; 53 had localized cutaneous leishmaniasis (LCL), and 7 had mucosal leishmaniasis (ML). Patients were treated at the Evandro Chagas Institute's leishmaniasis clinic, Pará State, Brazil. Ten healthy individuals with no history of ACL (control group) were also examined. Leishmania (V.) braziliensis promastigote antigen (PRO) was used to compare the reactivity with that of AMA antigen. Paired Student's t-test, kappa agreement, and Spearman test were used to evaluate the reactivity of AMA and PRO.

Results: The mean reactivity of AMA in ACL patients was 19.4 mm ± 13.3, which was higher (P < 0.001) than that of PRO: 12.1 mm ± 8.1. MST reactivity according to the clinical forms revealed that AMA reactivity in LCL and ML, 18.8 mm ± 13.3 and 24.3 mm ± 13.7, was higher (P < 0.001) than that of PRO, 11.8 mm ± 8.2 and 14.6 mm ± 8.4, respectively.

Conclusion: AMA reactivity was higher than that of PRO, indicating that AMA is a promising alternative for optimizing MST in the laboratory diagnosis of ACL.

查看原文本刊更多论文

事实证明，利什曼病（Viannia）lainsoni axenic amastigote 的抗原反应性是优化黑山皮试的合适替代品。

背景：美洲皮肤利什曼病（ACL）的实验室诊断需要一种适合巴西ACL流行病学状况的工具。黑山皮试（MST）是用于实验室诊断美国利什曼病的有效免疫学工具，它能诱导对利什曼原虫抗原的迟发型超敏反应（DTH）；然而，人类对感染的免疫反应受寄生虫非主原体的调节。Leishmania (V.) lainsoni能诱导人类产生强大的细胞免疫；因此，我们评估了其轴突状非主流体（AMA抗原）对MST的抗原反应性，以用于ACL的实验室诊断：在接受检查的 70 人中，60 人经实验室确诊为 ACL；53 人患有局部皮肤利什曼病（LCL），7 人患有粘膜利什曼病（ML）。患者在巴西帕拉州埃万德罗-查格斯研究所利什曼病诊所接受治疗。此外，还对 10 名无 ACL 病史的健康人（对照组）进行了检查。使用巴西利什曼原体抗原（PRO）来比较与AMA抗原的反应性。采用配对学生 t 检验、kappa 一致检验和 Spearman 检验来评估 AMA 和 PRO 的反应性：前交叉韧带损伤患者的平均 AMA 反应性为 19.4 mm ± 13.3，高于前交叉韧带损伤患者的 AMA 反应性（P 结论：前交叉韧带损伤患者的 AMA 反应性高于前交叉韧带损伤患者的 PRO 反应性：AMA的反应性高于PRO，这表明在前交叉韧带的实验室诊断中，AMA是优化MST的一种很有前途的替代方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Parasites & Vectors 医学-寄生虫学

CiteScore

6.30

自引率

9.40%

发文量

433

审稿时长

1.4 months

期刊介绍： Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.